NDLI: Cytogenetic and molecular evidence for an additional new species within the taxon Anopheles barbirostris (Diptera: Culicidae) in Thailand

Content Provider	Springer Nature Link
Author	Suwannamit, Sittiporn Baimai, Visut Otsuka, Yasushi Saeung, Atiporn Thongsahuan, Sorawat Tuetun, Benjawan Apiwathnasorn, Chamnarn Jariyapan, Narissara Somboon, Pradya Takaoka, Hiroyuki Choochote, Wej
Copyright Year	2008
Abstract	ITS2 DNA sequences of 42 isoline colonies of Anopheles barbirostris species A1 and A2 were analyzed and a new genetic species, temporarily designated as species A4 (Chiang Mai), was revealed. The large sequence divergences of the ITS2 (0.116-0.615), COI (0.023–0.048), and COII (0.030–0.040) genes between A. barbirostris species A4/A1 (Chiang Mai), A4/A2 (Phetchaburi), A4/A3 (Kanchanaburi), and A4/Anopheles campestris-like Form E (Chiang Mai) provided good supporting evidence. Species A1, A2, A3, and A4 share a mitotic karyotype of Form A (X1, X2, Y1). Crossing experiments between species A4 and the other four species yielded strong reproductive isolation producing few and/or non-hatched eggs and inviable and/or abnormal development of the reproductive system of F1 progenies. Moreover, available F1 hybrid larvae showed asynaptic polytene chromosome arms. Hence, molecular and cytogenetic evidence strongly support the existence of A. barbirostris species A4, which is more closely related to A. campestris-like Form E than to species A1, A2, and A3. Additionally, crossing experiments among 12 and seven isolines of different cytological forms of species A1 (A, B, C, D) and A2 (A, B), respectively, yielded fertile and viable F1 progenies. Thus, different karyotypic forms occurring in natural populations of species A1 and A2 merely represent intraspecies variation of sex chromosomes due to the extra blocks of heterochromatin.
Starting Page	905
Ending Page	918
Page Count	14
File Format	PDF
ISSN	09320113
Journal	Parasitology Research
Volume Number	104
Issue Number	4
e-ISSN	14321955
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-11-29
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Immunology Microbiology Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Veterinary Parasitology Insect Science

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