NDLI: Infectivity, predilection sites, and freeze tolerance of Trichinella spp. in experimentally infected sheep

Content Provider	Springer Nature Link
Author	Theodoropoulos, G. Kapel, C. M. O. Webster, P. Saravas, L. Zaki, J. Koutsotolis, K.
Copyright Year	2000
Abstract	A total of 36 sheep in groups of 4 were inoculated with 9 isolates of Trichinella and euthanized after 10 weeks. Thereafter, numbers of muscle larvae were determined in 13 different muscles/muscle groups. Muscle larvae were found in high numbers in all four sheep inoculated with T. spiralis, in lower numbers in two sheep inoculated with T. pseudospiralis (USA isolate), and in very low numbers in one sheep inoculated with T. pseudospiralis (USSR isolate) and one inoculated with T. britovi. In infections of high and moderate larval intensity, predilection sites of T. spiralis were the masseter muscles, the tongue, and the diaphragm and those of T. pseudospiralis were the masseter muscle and the neck. In low-intensity infections, muscle larvae were detected only in the diaphragm or in pooled muscle samples. For evaluation of the freeze tolerance of the different Trichinella species in sheep-muscle tissue, samples taken from the filet were stored at +5°, −5°, and −18 °C, respectively. After exposure for 1 and 4 weeks the tissue was digested and the released larvae were inoculated into mice for determination of the reproductive capacity index (RCI). Larvae of both T. spiralis and T. pseudospiralis survived freezing at −5° and −18 °C for 4 weeks.
Starting Page	401
Ending Page	405
Page Count	5
File Format	PDF
ISSN	09320113
Journal	Parasitology Research
Volume Number	86
Issue Number	5
e-ISSN	14321955
Language	English
Publisher	Springer-Verlag
Publisher Date	2000-04-25
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Veterinary Parasitology Insect Science

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