NDLI: Early response gene expression during differentiation of cultured Leishmania donovani

Content Provider	Springer Nature Link
Author	Duncan, Robert Alvarez, Raymond Jaffe, Charles L. Wiese, Martin Klutch, Michael Shakarian, Alison Dwyer, Dennis Nakhasi, Hira L.
Copyright Year	2001
Abstract	The promastigote form of the unicellular parasite, Leishmania donovani, must differentiate into the amastigote form to establish an infection in a mammalian host. Identification of genes whose expression changes during differentiation could help reveal mechanisms of Leishmania gene regulation and identify targets for controlling the diseases caused by this human pathogen. Two genomic clones were isolated, P9 that is more highly expressed in promastigotes than in axenic amastigotes and A14 that is preferentially expressed in axenic amastigotes. Analysis of the DNA sequences revealed open reading frames that would encode 55.5 kDa and 100 kDa proteins, respectively, with no homology to known proteins. The mRNA level for these genes during 24 h time courses of parasite differentiation in culture was compared to two genes known to be differentially expressed, c-lpk2 and mkk. Changes in RNA level occurred within 2 h for each gene and continued in advance of morphological changes. The expression levels of these four genes in axenic amastigotes correlated with results from animal-derived parasites.
Starting Page	897
Ending Page	906
Page Count	10
File Format	PDF
ISSN	09320113
Journal	Parasitology Research
Volume Number	87
Issue Number	11
e-ISSN	14321955
Language	English
Publisher	Springer-Verlag
Publisher Date	2001-07-10
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Veterinary Parasitology Insect Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Resistance to arsenite modulates expression of β- and γ-tubulin and sensitivity to paclitaxel during differentiation of Leishmania donovani

Testosterone attenuates p38 MAPK pathway during Leishmania donovani infection of macrophages

Biochemical alterations in paromomycin-treated Leishmania donovani promastigotes

Effect of testosterone on Leishmania donovani infection of macrophages

Expression and processing of megasome cysteine proteinases during Leishmania amazonensis differentiation

Comparison of the expression profiles of promastigotes and axenic amastigotes in Leishmania donovani using serial analysis of gene expression

In vitro sensitivity of Leishmania donovani to organometallic derivatives of pentamidine

Comparative transcript expression analysis of miltefosine-sensitive and miltefosine-resistant Leishmania donovani

Histopathologic analysis of hamster hepatocytes submitted to experimental infection with Leishmania donovani

Early response gene expression during differentiation of cultured Leishmania donovani

Similar Documents

Resistance to arsenite modulates expression of β- and γ-tubulin and sensitivity to paclitaxel during differentiation of Leishmania donovani

Testosterone attenuates p38 MAPK pathway during Leishmania donovani infection of macrophages

Biochemical alterations in paromomycin-treated Leishmania donovani promastigotes

Effect of testosterone on Leishmania donovani infection of macrophages

Expression and processing of megasome cysteine proteinases during Leishmania amazonensis differentiation

Comparison of the expression profiles of promastigotes and axenic amastigotes in Leishmania donovani using serial analysis of gene expression

In vitro sensitivity of Leishmania donovani to organometallic derivatives of pentamidine

Comparative transcript expression analysis of miltefosine-sensitive and miltefosine-resistant Leishmania donovani

Histopathologic analysis of hamster hepatocytes submitted to experimental infection with Leishmania donovani

Early response gene expression during differentiation of cultured Leishmania donovani