NDLI: The morphological analysis of autophagy in primary skeletal muscle cells infected with Toxoplasma gondii

Content Provider	Springer Nature Link
Author	Souto, Xênia Macedo Barbosa, Helene Santos Menna Barreto, Rubem Figueiredo Sadok
Copyright Year	2016
Abstract	Toxoplasma gondii is an obligate intracellular protozoan parasite, the causative agent of toxoplasmosis, one of the most widespread zoonoses in the world. During the host immune response, tissue cysts are formed, allowing the maintenance of the parasite within the host cell. Autophagy, a degradation process of cellular components, is critical for cellular homeostasis. Recently, it has been proposed that autophagy participates in host-pathogen interactions. Autophagic inducers (rapamycin or glucose plus serum deprivation) inhibited infection and parasite proliferation in a clinically relevant model of primary skeletal muscle cells (SkMC). The ultrastructural analysis showed in SkMC submitted to autophagic stimuli the presence of structures suggestive of autophagosomes close to the parasitophorous vacuole containing degraded parasites. Fluorescence microscopy results pointed out the increase in LC3 puncta in these cells after incubation with autophagic inducers. In the present study, SkMC autophagy controlled the proliferation of tachyzoites inside the cell, data reinforced by ultrastructural evidences and increased LC3 expression.
Starting Page	2853
Ending Page	2861
Page Count	9
File Format	PDF
ISSN	09320113
Journal	Parasitology Research
Volume Number	115
Issue Number	7
e-ISSN	14321955
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-04-14
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Toxoplasma gondii Autophagy Skeletal muscle cells Electron microscopy LC3 puncta Medical Microbiology Microbiology Immunology
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Veterinary Parasitology Insect Science

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