NDLI: Involvement of signaling through protein kinase C and phosphatidylinositol 3-kinase in the excystation and metacystic development of Entamoeba invadens

Content Provider	Springer Nature Link
Author	Makioka, Asao Kumagai, Masahiro Kobayashi, Seiki Takeuchi, Tsutomu
Copyright Year	2003
Abstract	Using an axenic excystation system in vitro, we examined the effect of protein kinase C (PKC) and phosphatidylinositol 3-kinase (PI3K), which are signaling molecules responsible for numerous cellular responses, on the excystation and metacystic development of Entamoeba invadens. Excystation, which was assessed by counting the number of metacystic amoebae after the induction of excystation, was inhibited by the PKC inhibitors staurosporine, chelerythrine chloride and calphostin C in a concentration-dependent manner during incubation, compared with the controls. As cyst viability was not affected by these inhibitors, reduced excystation was not due to their direct toxic effects on cysts. Metacystic development, when determined by the number of nuclei in the amoebae, was delayed by these PKC inhibitors, because the percentage of 1-nucleate amoebae was lower than in controls at day 3 of incubation. Wortmannin, a potent inhibitor of PI3K, also inhibited excystation and metacystic development of E. invadens in a concentration-dependent manner, compared with the controls. These results indicate that signaling through PKC and PI3K contributes to the excystation and metacystic development of E. invadens.
Starting Page	204
Ending Page	208
Page Count	5
File Format	PDF
ISSN	09320113
Journal	Parasitology Research
Volume Number	91
Issue Number	3
e-ISSN	14321955
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-08-16
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Veterinary Parasitology Insect Science

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Involvement of serine proteases in the excystation and metacystic development of Entamoeba invadens

Involvement of serine proteases in the excystation and metacystic development of Entamoeba invadens

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