NDLI: Development of a facile system for mass production of Brugia malayi in a small-space laboratory

Content Provider	Springer Nature Link
Author	Saeung, Atiporn Choochote, Wej
Copyright Year	2013
Abstract	Brugia malayi is one of the important lymphatic filarial nematodes that cause elephantiasis and disability in humans in the Asian region. Mass production at any stage of this nematode in both small laboratory animal hosts and mosquito vectors is still necessary in order to continue various research aspects. This study elucidated on the use of nonblood feeding or the autogenous Ochlerotatus togoi (Thailand strain) and male Mongolian jird (Meriones unguiculatus) system. This has brought about a low-cost and highly-effective procedure for the mass production of blood containing microfilariae, infective (L3) larvae, and adults of B. malayi under nonanimal-blood-feeding insectary and small-space animal-house conditions. The highly-infective rates (human-heparinized blood, 86.67–93.33; swine-heparinized blood, 83.33–96.67; bovine-heparinized blood, 76.67–80; chicken-heparinized blood, 73.33–76.67) and parasite loads (human-heparinized blood, 10.58–12.36; swine-heparinized blood, 8.40–10.38; bovine-heparinized blood, 9.75–9.91; chicken-heparinized blood, 3.41–4.65) of autogenous O. togoi to B. malayi and high numbers of adults recovered from ten B. malayi-infected male jirds (total = 327, 16–52) are good supportive evidence. In addition, all special techniques required for succeeding in the establishment of a facile system regarding these matters are detailed.
Starting Page	3259
Ending Page	3265
Page Count	7
File Format	PDF
ISSN	09320113
Journal	Parasitology Research
Volume Number	112
Issue Number	9
e-ISSN	14321955
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-07-03
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Medical Microbiology Microbiology Immunology
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Veterinary Parasitology Insect Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Intraperitoneal development of the filarial nematode Brugia malayi in the Mongolian jird (Meriones unguiculatus)

Translationally controlled tumor protein of Brugia malayi functions as an antioxidant protein

Exsheathment and midgut invasion of nocturnally subperiodic Brugia malayi microfilariae in a refractory vector, Aedes aegypti (Thailand strain)

Molecular cloning, purification and characterisation of myosin of human lymphatic filarial parasite Brugia malayi

The role of Brugia malayi ATP-binding cassette (ABC) transporters in potentiating drug sensitivity

Molecular characterization of a Brugia malayi transglutaminase

Molecular cloning and characterization of a novel immunoreactive ATPase/RNA helicase in human filarial parasite Brugia malayi

Identification and characterization of microRNAs in Trichinella spiralis by comparison with Brugia malayi and Caenorhabditis elegans

Improved antifilarial activity of ivermectin in chitosan–alginate nanoparticles against human lymphatic filarial parasite, Brugia malayi

Development of a facile system for mass production of Brugia malayi in a small-space laboratory

Similar Documents

Intraperitoneal development of the filarial nematode Brugia malayi in the Mongolian jird (Meriones unguiculatus)

Translationally controlled tumor protein of Brugia malayi functions as an antioxidant protein

Exsheathment and midgut invasion of nocturnally subperiodic Brugia malayi microfilariae in a refractory vector, Aedes aegypti (Thailand strain)

Molecular cloning, purification and characterisation of myosin of human lymphatic filarial parasite Brugia malayi

The role of Brugia malayi ATP-binding cassette (ABC) transporters in potentiating drug sensitivity

Molecular characterization of a Brugia malayi transglutaminase

Molecular cloning and characterization of a novel immunoreactive ATPase/RNA helicase in human filarial parasite Brugia malayi

Identification and characterization of microRNAs in Trichinella spiralis by comparison with Brugia malayi and Caenorhabditis elegans

Improved antifilarial activity of ivermectin in chitosan–alginate nanoparticles against human lymphatic filarial parasite, Brugia malayi

Development of a facile system for mass production of Brugia malayi in a small-space laboratory