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| Content Provider | Springer Nature Link |
|---|---|
| Author | Wang, Jin Lei Huang, Si Yang Li, Ting Ting Chen, Kai Ning, Hong Rui Zhu, Xing Quan |
| Copyright Year | 2015 |
| Abstract | Toxoplasma gondii, an important protozoan parasite, infects almost all warm-blooded animals and humans. Although treatments in T. gondii are limited by the lack of effective drugs, some calcium-dependent kinases were demonstrated as the promising drug targets to chemotherapy against T. gondii due to their essential roles in T. gondii and absence from their hosts. The objectives of the present study were to investigate the functions of six calcium-dependent protein kinases (CDPK4, CDPK4A, CDPK5, CDPK6, CDPK8, and CDPK9) in T. gondii to assess whether they are suitable for designing as drug targets. We used the CRISPR-Cas9 system to disrupt six CDPK genes successfully by insertion of DHFR* at the guide RNA-targeted region in the six endogenous CDPK loci and successfully obtained the six knockout (KO)-CDPK strains. The biological characteristics of the six strains were evaluated by plaque assays, invasion, egress, replication, and virulence assays, respectively. The results indicated that there was no significant difference between the six KO-CDPK strains and wild-type strain in virulence and the lytic cycle including invasion, egress, and replication. The conclusion was the six CDPKs are not essential for T. gondii lytic cycle and also not virulence factors for mice, suggesting that the six CDPKs may participate in other functions in T. gondii. |
| Starting Page | 697 |
| Ending Page | 702 |
| Page Count | 6 |
| File Format | |
| ISSN | 09320113 |
| Journal | Parasitology Research |
| Volume Number | 115 |
| Issue Number | 2 |
| e-ISSN | 14321955 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2015-10-26 |
| Publisher Place | Berlin/Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Toxoplasma gondii Calcium-dependent protein kinases (CDPKs) CRISPR-Cas9 system Gene functions Medical Microbiology Microbiology Immunology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Infectious Diseases Veterinary Parasitology Insect Science |
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