NDLI: Cell invasion by Trypanosoma cruzi amastigotes of distinct infectivities: studies on signaling pathways

Content Provider	Springer Nature Link
Author	Fernandes, Adriana B. Neira, Ivan Ferreira, Alice T. Mortara, Renato A.
Copyright Year	2006
Abstract	Trypanosoma cruzi metacyclic trypomastigotes of the major phylogenetic lineages use specific signaling pathways to invade host cells. Using a panel of drugs, we studied if the differences in the ability of extracellular amastigotes (EA) from G (T. cruzi I) and CL (T. cruzi II) strains to invade host cells could be associated to activation of specific signaling routes. Sonicated extracts from G or CL strain EA induced transient raises in HeLa cell intracellular Ca2+ levels in a dose-dependent manner. Treatment of EA with drugs that affect Ca2+ release from inositol-1,4,5-triphosphate-sensitive stores did not significantly affect the infectivity of either strain, whereas EA of both strains treated with ionomycin plus NH4Cl or nigericin that release Ca2+ from acidocalcisomes had their infectivity reduced. Treatment of parasites with adenylate cyclase activator forskolin increased the infectivity of both strains towards HeLa cells. These data, taken together, suggest that, for host cell invasion, G and CL strain EA engage signaling pathways that lead to an increase of cyclic adenosine monophosphate and Ca2+ mobilization from acidocalcisomes. Moreover, treatment of EA with genistein reduced by ∼45% the invasion of HeLa cells by G but not by CL strain, implicating a protein tyrosine kinase in the process. In line with this, HeLa cell extracts contained a protein tyrosine kinase activity that mediated the phosphorylation of 87- and 175-kDa polypeptides of EA from G but not from CL strain. Regarding the target cell response, the activation of host PI3 kinase appears to be required for invasion by either strain as treatment of HeLa cells with wortmannin reduced EA infectivity. These data overall reinforce the concept that cell invasion by T. cruzi EA markedly differs from the process involving metacyclic trypomastigotes.
Starting Page	59
Ending Page	68
Page Count	10
File Format	PDF
ISSN	09320113
Journal	Parasitology Research
Volume Number	100
Issue Number	1
e-ISSN	14321955
Language	English
Publisher	Springer-Verlag
Publisher Date	2006-06-22
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Immunology Microbiology Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Veterinary Parasitology Insect Science

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