NDLI: Effects of hypotonicity on amino acid release in adult Echinostoma caproni as determined by thin layer chromatography

Content Provider	Springer Nature Link
Author	Ponder, Elizabeth L. Fried, Bernard Sherma, Joseph
Copyright Year	2003
Abstract	Thin layer chromatography (TLC) was used to analyze the amino acids in worm incubates isotonic and hypotonic to the intestinal habitat of adult Echinostoma caproni and to analyze the free pool amino acids of these trematodes after incubation. Qualitative analysis revealed the presence of histidine, lysine, alanine, and proline in all samples of incubate and worm tissue. Quantification of histidine and lysine by TLC with densitometry gave mean concentrations of 24.1 µg histidine/g worm per ml incubate in Locke's solution and 195.0 µg lysine/g worm per ml incubate in deioinized (DI) water. Quantification of histidine and lysine in the worm tissue gave mean weight percents of 0.0587 and 0.0263, respectively, in worms incubated in Locke's solution and 0.0229 and 0.0163, respectively, for worms incubated in DI water. Our findings suggest that E. caproni adults may leak amino acids for osmoregulation in hypotonic environments.
Starting Page	242
Ending Page	244
Page Count	3
File Format	PDF
ISSN	09320113
Journal	Parasitology Research
Volume Number	89
Issue Number	3
e-ISSN	14321955
Language	English
Publisher	Springer-Verlag
Publisher Date	2002-11-06
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Veterinary Parasitology Insect Science

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Effects of hypotonicity on amino acid release in adult Echinostoma caproni as determined by thin layer chromatography