NDLI: Multilocus genotype and subtype analysis of Cryptosporidium andersoni derived from a Bactrian camel (Camelus bactrianus) in China

Content Provider	Springer Nature Link
Author	Liu, Xuehan Zhou, Xiaoxiao Zhong, Zhijun Deng, Jiabo Chen, Weigang Cao, Suizhong Fu, Hualin Zuo, Zhicai Hu, Yanchun Peng, Guangneng
Copyright Year	2014
Abstract	Fecal specimens from two Bactrian camels were collected in the Ya’an city zoo of China and were examined for Cryptosporidium by centrifugal flotation. One specimen was found to be parasitized by Cryptosporidium via microscopy, and the oocysts were measured to have an average size of 7.03 × 5.50 μm (n > 50). The isolate was genotyped by polymerase chain reaction (PCR) amplification and DNA sequence analysis of the partial 18S rRNA, COWP, and A135 genes, and was confirmed to be Cryptosporidium andersoni with minor nucleotide differences. Multilocus sequence typing (MLST) analysis indicated that the subtype of the camel-derived C. andersoni isolate was A4, A4, A4, and A1 at the four minisatellite loci (MS1, MS2, MS3, and MS16, respectively). Therefore, this isolate belongs to the most common MLST subtype reported in cattle in China and is distinct from two other known camel C. andersoni MLST subtypes (A6, A4, A2, A1 and A6, A5, A2, A1). Animal transmission experiments demonstrated that the C. andersoni isolate was not infectious to immunosuppressed or immunocompetent Kun-ming mice, Sprague-Dawley rats, and hamsters but was biologically similar to most bovine C. andersoni isolates characterized so far. Therefore, transmission of this camel-derived C. andersoni isolate is very likely to occur between camels and bovine.
Starting Page	2129
Ending Page	2136
Page Count	8
File Format	PDF
ISSN	09320113
Journal	Parasitology Research
Volume Number	113
Issue Number	6
e-ISSN	14321955
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-03-28
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cryptosporidium C. andersoni Bactrian camel 18S rRNA Cross-transmission MLST subtype Medical Microbiology Microbiology Immunology
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Veterinary Parasitology Insect Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Multilocus phylogenetic analysis of Cryptosporidium andersoni (Apicomplexa) isolated from a bactrian camel (Camelus bactrianus) in China

Molecular identification and biological characterization of Cryptosporidium muris from camels (Camelus bactrianus) in China

Host specificity of Enterocytozoon bieneusi genotypes in Bactrian camels (Camelus bactrianus) in China

Prevalence of Cryptosporidium infection in captive lesser panda (Ailurus fulgens) in China

Anaplasma infection of Bactrian camels (Camelus bactrianus) and ticks in Xinjiang, China

The first report of Cryptosporidium andersoni in horses with diarrhea and multilocus subtype analysis

Genetic Diversity of Cryptosporidium in Bactrian Camels (Camelus bactrianus) in Xinjiang, Northwestern China

Morphology of Rhinencephalon and Hippocampal formation of the Bactrian Camel (Camelus bactrianus) with their adaptive features

Novel Acanthamoeba 18S rRNA gene sequence type from an environmental isolate

Multilocus genotype and subtype analysis of Cryptosporidium andersoni derived from a Bactrian camel (Camelus bactrianus) in China

Similar Documents

Multilocus phylogenetic analysis of Cryptosporidium andersoni (Apicomplexa) isolated from a bactrian camel (Camelus bactrianus) in China

Molecular identification and biological characterization of Cryptosporidium muris from camels (Camelus bactrianus) in China

Host specificity of Enterocytozoon bieneusi genotypes in Bactrian camels (Camelus bactrianus) in China

Prevalence of Cryptosporidium infection in captive lesser panda (Ailurus fulgens) in China

Anaplasma infection of Bactrian camels (Camelus bactrianus) and ticks in Xinjiang, China

The first report of Cryptosporidium andersoni in horses with diarrhea and multilocus subtype analysis

Genetic Diversity of Cryptosporidium in Bactrian Camels (Camelus bactrianus) in Xinjiang, Northwestern China

Morphology of Rhinencephalon and Hippocampal formation of the Bactrian Camel (Camelus bactrianus) with their adaptive features

Novel Acanthamoeba 18S rRNA gene sequence type from an environmental isolate

Multilocus genotype and subtype analysis of Cryptosporidium andersoni derived from a Bactrian camel (Camelus bactrianus) in China