NDLI: Galactose-specific adhesin and cytotoxicity of Entamoeba histolytica

Content Provider	Springer Nature Link
Author	López Vancell, Rosario Montfort, Irmgard Pérez Tamayo, Ruy
Copyright Year	2000
Abstract	We examined the molecular mechanisms of the cytotoxicity of Entamoeba histolytica, using the loss of transepithelial electrical resistance (TER) of monolayers of Madin-Darby canine-kidney (MDCK) cells on their incubation with axenic trophozoites of the HM1-IMSS strain. Such loss of TER occurs very early (in 2–5 min) and is caused by the opening of tight junctions and the detachment of cells. We used specific inhibitors for three of the four molecules currently accepted as being responsible for cytotoxicity: galactose-specific adhesin(s), phospholipase A, and cysteine proteinases. We also used inhibitors of calcium channels. Axenic trophozoites of E. histolytica strain HM1-IMSS were preincubated with the different inhibitors for 1 h prior to their coincubation with MDCK-cell monolayers. The only inhibitor that effectively blocked the loss of TER caused by the parasite was galactose. We suggest that in this experimental model, galactose-specific adhesin(s) are essential for amebic cytotoxicity.
Starting Page	226
Ending Page	231
Page Count	6
File Format	PDF
ISSN	09320113
Journal	Parasitology Research
Volume Number	86
Issue Number	3
e-ISSN	14321955
Language	English
Publisher	Springer-Verlag
Publisher Date	2000-02-18
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Veterinary Parasitology Insect Science

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