NDLI: Real-time PCR method for the detection and quantification of Acanthamoeba species in various types of water samples

Content Provider	Springer Nature Link
Author	Kao, Po Min Tung, Min Che Hsu, Bing Mu Tsai, Hsien Lung She, Cheng Yu Shen, Shu Min Huang, Wen Chien
Copyright Year	2013
Abstract	In this study, a quantitative real-time PCR was developed to detect and quantify Acanthamoeba spp. in various environmental water samples. The water samples were taken from watershed, water treatment plant, and three thermal spring recreation areas. The overall detection rate was 14.2 % (25/176) for Acanthamoeba spp. The percentages of samples containing Acanthamoeba spp. from river water, raw drinking water, and thermal spring water were 13 % (13/100), 25 % (7/28), and 10.4 % (5/48), respectively. Acanthamoeba spp. concentrations were determined according to SYBR Green quantitative real-time PCR. A plasmid-based standard curve was constructed to determine the Acanthamoeba concentration using dilution factors for achieving 1.36 × 109 gene copies per PCR for 18S rRNA gene in Acanthamoeba spp. The resulting concentrations varied by the type of water, in the range of 46–2.6 × 102 cells/l in positive raw drinking water, 2.7 × 102–1.5 × 104 cells/l in river water, and 54–1.7 × 103 cells/l in thermal spring water. The presence of Acanthamoeba spp. in the raw drinking water samples was also found to have a significant difference with heterotrophic plate count. The presence of Acanthamoeba spp. in various aquatic environments may be a potential health hazard and must be further evaluated.
Starting Page	1131
Ending Page	1136
Page Count	6
File Format	PDF
ISSN	09320113
Journal	Parasitology Research
Volume Number	112
Issue Number	3
e-ISSN	14321955
Language	English
Publisher	Springer-Verlag
Publisher Date	2013-01-10
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Medical Microbiology Microbiology Immunology
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Veterinary Parasitology Insect Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Quantitative detection and identification of Naegleria spp. in various environmental water samples using real-time quantitative PCR assay

Detection of Blastocystis from stool samples using real-time PCR

Evaluation of a molecular beacon real-time PCR assay for detection of Baylisascaris procyonis in different soil types and water samples

A simple PCR condition for detection of a single cyst of Acanthamoeba species

The first genotype determination of Acanthamoeba potential threat to human health, isolated from natural water reservoirs in Poland

Comparison of real-time PCR methods for the detection of Naegleria fowleri in surface water and sediment

Detection and characterization of Cryptosporidium cuniculus by real-time PCR

A simple method for long-term storage of Acanthamoeba species

Real-time PCR assay targeting the actin gene for the detection of Cryptosporidium parvum in calf fecal samples

Real-time PCR method for the detection and quantification of Acanthamoeba species in various types of water samples

Similar Documents

Quantitative detection and identification of Naegleria spp. in various environmental water samples using real-time quantitative PCR assay

Detection of Blastocystis from stool samples using real-time PCR

Evaluation of a molecular beacon real-time PCR assay for detection of Baylisascaris procyonis in different soil types and water samples

A simple PCR condition for detection of a single cyst of Acanthamoeba species

The first genotype determination of Acanthamoeba potential threat to human health, isolated from natural water reservoirs in Poland

Comparison of real-time PCR methods for the detection of Naegleria fowleri in surface water and sediment

Detection and characterization of Cryptosporidium cuniculus by real-time PCR

A simple method for long-term storage of Acanthamoeba species

Real-time PCR assay targeting the actin gene for the detection of Cryptosporidium parvum in calf fecal samples

Real-time PCR method for the detection and quantification of Acanthamoeba species in various types of water samples