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| Content Provider | Springer Nature Link |
|---|---|
| Author | Delfani, Somayeh Mohabati Mobarez, Ashraf Imani Fooladi, Abbas Ali Amani, Jafar Emaneini, Mohammad |
| Copyright Year | 2015 |
| Abstract | Staphylococcus aureus is one of the most important causes of nosocomial infections. An effective vaccine to prevent S. aureus infections is urgently required due to the dramatic increase in the number of antibiotic-resistant strains. In this report, we evaluated a newly recombinant protein composed of selected antigenic regions of clumping factor A (ClfA), iron surface determinant B (IsdB) and gamma hemolysin B (HlgB) of S. aureus and sequence coding for hydrophobic linkers between three domains. The recombinant gene was constructed in pET-28a (+) and expressed in Escherichia coli BL21. In addition, sequence coding for a His6-tag was added followed by a hybrid procedure of nickel chelate protein purification. Immunization of BALB/c mice with the recombinant protein ClfA–IsdB–Hlg evoked antigen-specific antibodies that could opsonize S. aureus cells, enhancing in vitro phagocytosis by macrophages. Vaccination with the recombinant protein also reduced the bacterial load recovered from mice spleen samples and increased survival following the intraperitoneal challenge with pathogenic S. aureus compared to the control mice. Our results showed that the recombinant protein ClfA–IsdB–Hlg is a promising vaccine candidate for the prevention of S. aureus bacteremia infections. |
| Starting Page | 47 |
| Ending Page | 55 |
| Page Count | 9 |
| File Format | |
| ISSN | 03008584 |
| Journal | Medical Microbiology and Immunology |
| Volume Number | 205 |
| Issue Number | 1 |
| e-ISSN | 14321831 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2015-07-09 |
| Publisher Place | Berlin/Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Staphylococcus aureus Clumping factor A (ClfA) Iron surface determinant B (IsdB) Gamma hemolysin B (HlgB) recombinant protein Immunogenicity Medical Microbiology Immunology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Immunology Microbiology (medical) |
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