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| Content Provider | Springer Nature Link |
|---|---|
| Author | Thomsen, Morten Skøtt Cinar, Betül Jensen, Majbrit Myrup Lyukmava, Ekaterina N. Shulepko, Mikhail A. Tsetlin, Victor Klein, Anders Bue Mikkelsen, Jens D. |
| Copyright Year | 2013 |
| Abstract | The Ly-6 superfamily of proteins, which affects diverse processes in the immune system, has attracted renewed attention due to the ability of some Ly-6 proteins to bind to and modulate the function of neuronal nicotinic acetylcholine receptors (nAChRs). However, there is a scarcity of knowledge regarding the distribution and developmental regulation of these proteins in the brain. We use protein cross-linking and synaptosomal fractions to demonstrate that the Ly-6 proteins Lynx1 and Ly6H are membrane-bound proteins in the brain, which are present on the cell surface and localize to synaptic compartments. We further estimate the amount of Lynx1 in the rat cortex using known amounts of a heterologously expressed soluble Lynx1 variant (ws-Lynx1) to be approximately 8.6 ng/μg total protein, which is in line with the concentrations of ws-Lynx1 required to affect nAChR function. In addition, we demonstrate that Lynx1 and Ly6H are expressed in cultured neurons, but not cultured micro- or astroglial cultures. In addition, Lynx1, but not Ly6H was detected in the CSF. Finally, we show that the Ly-6 proteins Lynx1, Lynx2, Ly6H, and PSCA, display distinct expression patterns during postnatal development in the rat frontal cortex and hippocampus at the mRNA and protein level, and that this is paralleled to some degree by the expression of the nAChR subunits α2, α4, α7 and β2. Our results demonstrate a developmental pattern, localization, and concentration of Ly-6 proteins in the brain, which support a role for these proteins in the modulation of signaling at synaptic membranes. |
| Starting Page | 1923 |
| Ending Page | 1934 |
| Page Count | 12 |
| File Format | |
| ISSN | 18632653 |
| Journal | Anatomy and Embryology |
| Volume Number | 219 |
| Issue Number | 6 |
| e-ISSN | 18632661 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2013-07-17 |
| Publisher Place | Berlin/Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Nicotinic acetylcholine receptor Allosteric modulator Frontal cortex Hippocampus Synaptosomes Lynx1 Lynx2 Ly6H PSCA Neurosciences Cell Biology Neurology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Neuroscience Anatomy Histology |
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