NDLI: Dissecting blue light signal transduction pathway in leaf epidermis using a pharmacological approach

Content Provider	Springer Nature Link
Author	Živavić, Branka D. Shabala, Lana I. Elzenga, Theo J. M. Shabala, Sergey N.
Copyright Year	2015
Abstract	Blue light signalling pathway in broad bean leaf epidermal cells includes key membrane transporters: plasma- and endomembrane channels and pumps of H + , Ca 2+ and K + ions, and plasma membrane redox system. Blue light signalling pathway in epidermal tissue isolated from the abaxial side of fully developed Vicia faba leaves was dissected by measuring the effect of inhibitors of second messengers on net K+, Ca2+ and H+ fluxes using non-invasive ion-selective microelectrodes (the MIFE system). Switching the blue light on–off caused transient changes of the ion fluxes. The effects of seven groups of inhibitors were tested in this study: CaM antagonists, ATPase inhibitors, Ca2+ anatagonists or chelators, agents affecting IP3 formation, redox system inhibitors, inhibitors of endomembrane Ca2+ transport systems and an inhibitor of plasma membrane Ca2+-permeable channels. Most of the inhibitors had a significant effect on steady-state (basal) net fluxes, as well as on the magnitude of the transient ion flux responses to blue light fluctuations. The data presented in this study suggest that redox signalling and, specifically, plasma membrane NADPH oxidase and coupled Ca2+ and K+ fluxes play an essential role in blue light signal transduction.
Starting Page	813
Ending Page	827
Page Count	15
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	242
Issue Number	4
e-ISSN	14322048
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-05-13
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Broad bean Inhibitors Ion fluxes Microelectrodes Plant Sciences Agriculture Ecology Forestry
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Ion transport in broad bean leaf mesophyll under saline conditions

Rice PLASTOCHRON genes regulate leaf maturation downstream of the gibberellin signal transduction pathway

Cell-to-cell pathway dominates xylem-epidermis hydraulic connection in Tradescantia fluminensis (Vell. Conc.) leaves

Dissection of heat-induced systemic signals: superiority of ion fluxes to voltage changes in substomatal cavities

Surface tip-to-base Ca2+ and H+ ionic fluxes are involved in apical growth and graviperception of the Phycomyces stage I sporangiophore

Signal transduction during wheat grain development

Are trichomes involved in the biomechanical systems of Cucurbita leaf petioles?

Systemic signalling in barley through action potentials

Aluminum inhibits phosphatidic acid formation by blocking the phospholipase C pathway

Dissecting blue light signal transduction pathway in leaf epidermis using a pharmacological approach

Similar Documents

Ion transport in broad bean leaf mesophyll under saline conditions

Rice PLASTOCHRON genes regulate leaf maturation downstream of the gibberellin signal transduction pathway

Cell-to-cell pathway dominates xylem-epidermis hydraulic connection in Tradescantia fluminensis (Vell. Conc.) leaves

Dissection of heat-induced systemic signals: superiority of ion fluxes to voltage changes in substomatal cavities

Surface tip-to-base Ca2+ and H+ ionic fluxes are involved in apical growth and graviperception of the Phycomyces stage I sporangiophore

Signal transduction during wheat grain development

Are trichomes involved in the biomechanical systems of Cucurbita leaf petioles?

Systemic signalling in barley through action potentials

Aluminum inhibits phosphatidic acid formation by blocking the phospholipase C pathway

Dissecting blue light signal transduction pathway in leaf epidermis using a pharmacological approach