NDLI: In vitro co-cultures of Pinus pinaster with Bursaphelenchus xylophilus: a biotechnological approach to study pine wilt disease

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Planta : Volume 241, Issue 5, May 2015

Planta : Volume 241, Issue 4, April 2015

Planta : Volume 241, Issue 3, March 2015

Planta : Volume 241, Issue 2, February 2015

Planta : Volume 241, Issue 1, January 2015

In vitro co-cultures of Pinus pinaster with Bursaphelenchus xylophilus: a biotechnological approach to study pine wilt disease

Content Provider	Springer Nature Link
Author	Faria, Jorge M. S. Sena, Inês Vieira da Silva, Inês Ribeiro, Bru Barbosa, Pedro Ascensão, Lia Bennett, Richard N. Mota, Manuel Figueiredo, A. Cristina
Copyright Year	2015
Abstract	Co-cultures of Pinus pinaster with Bursaphelenchus xylophilus were established as a biotechnological tool to evaluate the effect of nematotoxics addition in a host/parasite culture system. The pinewood nematode (PWN), Bursaphelenchus xylophilus, the causal agent of pine wilt disease (PWD), was detected for the first time in Europe in 1999 spreading throughout the pine forests in Portugal and recently in Spain. Plant in vitro cultures may be a useful experimental system to investigate the plant/nematode relationships in loco, thus avoiding the difficulties of field assays. In this study, Pinus pinaster in vitro cultures were established and compared to in vivo 1 year-old plantlets by analyzing shoot structure and volatiles production. In vitro co-cultures were established with the PWN and the effect of the phytoparasite on in vitro shoot structure, water content and volatiles production was evaluated. In vitro shoots showed similar structure and volatiles production to in vivo maritime pine plantlets. The first macroscopic symptoms of PWD were observed about 4 weeks after in vitro co-culture establishment. Nematode population in the culture medium increased and PWNs were detected in gaps of the callus tissue and in cavities developed from the degradation of cambial cells. In terms of volatiles main components, plantlets, P. pinaster cultures, and P. pinaster with B. xylophilus co-cultures were all β- and α-pinene rich. Co-cultures may be an easy-to-handle biotechnological approach to study this pathology, envisioning the understanding of and finding ways to restrain this highly devastating nematode.
Starting Page	1325
Ending Page	1336
Page Count	12
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	241
Issue Number	6
e-ISSN	14322048
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-02-13
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Maritime pine Monoxenic culture Pinewood nematode Relative water content Shoots structure Volatiles Plant Sciences Agriculture Ecology Forestry
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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Primary Metabolite Adjustments Associated With Pinewood Nematode Resistance in Pinus pinaster

In vitro co-cultures of Pinus pinaster with Bursaphelenchus xylophilus: a biotechnological approach to study pine wilt disease

Insights Into the Mechanisms Implicated in Pinus pinaster Resistance to Pinewood Nematode

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In vitro co-cultures of Pinus pinaster with Bursaphelenchus xylophilus: a biotechnological approach to study pine wilt disease

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