NDLI: Purification, characterization and physiological role of sucrose synthase in the pea seed coat (Pisum sativum L.)

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Planta : Volume 201, Issue 4, April 1997

Planta : Volume 201, Issue 3, March 1997

Planta : Volume 201, Issue 1, March 1997

Purification, characterization and physiological role of sucrose synthase in the pea seed coat (Pisum sativum L.)

Content Provider	Springer Nature Link
Author	Déjardin, A. Rochat, C. Maugenest, S. Boutin, J. P.
Copyright Year	1997
Abstract	The seed coat is a maternal organ which surrounds the embryo and is involved in the control of its nutrition. This study with pea (Pisum sativum L.) was conducted to understand more fully the sucrose/starch interconversions occurring in the seed coat. The concentrations of soluble sugars, the starch content, and the activities of the sucrose-metabolizing enzymes, sucrose synthase (Sus; EC 2.4.1.13), alkaline and soluble acid invertase (EC 3.2.1.26) and sucrose-phosphate synthase (SPS; EC 2.4.1.14) were compared at four developmental stages during seed filling. Among the four enzymes, only Sus activity was very high and strongly correlated with the starch concentration in the seed coat. Sucrose synthase catalyses the cleavage of sucrose in the presence of UDP into UDP-glucose and fructose. Sucrose synthase was purified from pea seed coats in a three-step protocol, consisting of diethylaminoethyl-Sephacel chromatography, gel filtration and affinity chromatography. The enzyme was characterized at the biochemical and molecular levels. Sucrose synthase exhibits biochemical properties which allow it to function in the direction of both sucrose cleavage and synthesis. The mass-action ratio of its four substrates was close to the theoretical equilibrium constant at the four developmental stages we studied. A labelling experiment on seed coats has shown that Sus activity is reversible in vivo and can produce 37% of neosynthesized sucrose in the seed coat cells (minimum value). It is concluded that Sus could play a central role in the control of sucrose concentration in the seed coat cells in response to the demand for sucrose in the embryo during the development of the seed.
Starting Page	128
Ending Page	137
Page Count	10
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	201
Issue Number	2
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	1997-01-01
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Pisum Seed coat Sucrose Sucrose-metabolizing enzymes Sucrose synthase Starch Agriculture Ecology Forestry Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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Purification, characterization and physiological role of sucrose synthase in the pea seed coat (Pisum sativum L.)