NDLI: The 3′ untranslated region of the two cytosolic glutamine synthetase (GS1) genes in alfalfa (Medicago sativa) regulates transcript stability in response to glutamine

Content Provider	Springer Nature Link
Author	Simon, Bindu Sengupta Gopalan, Champa
Copyright Year	2010
Abstract	Glutamine synthetase (GS) catalyzes the ATP-dependent condensation of ammonia with glutamate to produce glutamine. The GS enzyme is located either in the chloroplast (GS2) or in the cytoplasm (GS1). GS1 is encoded by a small gene family and the members exhibit differential expression pattern mostly attributed to transcriptional regulation. Based on our recent finding that a soybean GS1 gene, Gmglnβ 1 is subject to its 3′UTR-mediated post-transcriptional regulation as a transgene in alfalfa (Medicago sativa) we have raised the question of whether the 3′UTR-mediated transcript destabilization is a more universal phenomenon. Gene constructs consisting of the CaMV35S promoter driving the reporter gene, GUS, followed by the 3′UTRs of the two alfalfa GS1 genes, MsGSa and MsGSb, were introduced into alfalfa and tobacco. The analysis of these transformants suggests that while both the 3′UTRs promote transcript turnover, the MsGSb 3′UTR is more effective than the MsGSa 3′UTR. However, both the 3′UTRs along with Gmglnβ 1 3′UTR respond to nitrate as a trigger in transcript turnover. More detailed analysis points to glutamine rather than nitrate as the mediator of transcript turnover. Our data suggests that the 3′UTR-mediated regulation of GS1 genes at the level of transcript turnover is probably universal and is used for fine-tuning the expression in keeping with the availability of the substrates.
Starting Page	1151
Ending Page	1162
Page Count	12
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	232
Issue Number	5
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-08-13
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Glutamine synthetase Medicago sativa Alfalfa 3′ Untranslated region Post-transcriptional regulation Nitrogen assimilation Glutamine Ecology Forestry Agriculture Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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The 3′ untranslated region of a soybean cytosolic glutamine synthetase $(GS_{1}$) affects transcript stability and protein accumulation in transgenic alfalfa

The 3′ untranslated region of a soybean cytosolic glutamine synthetase (GS1) affects transcript stability and protein accumulation in transgenic alfalfa

Impact of concurrent overexpression of cytosolic glutamine synthetase (GS1) and sucrose phosphate synthase (SPS) on growth and development in transgenic tobacco

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Constitutive overexpression of cytosolic glutamine synthetase (GS1) gene in transgenic alfalfa demonstrates that GS1 may be regulated at the level of RNA stability and protein turnover.

Constitutive Overexpression of Cytosolic Glutamine Synthetase (GS1) Gene in Transgenic Alfalfa Demonstrates That GS1 May Be Regulated at the Level of RNA Stability and Protein Turnover1

The 3′ untranslated region of the two cytosolic glutamine synthetase (GS1) genes in alfalfa (Medicago sativa) regulates transcript stability in response to glutamine

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