NDLI: Characterization of Al-responsive citrate excretion and citrate-transporting MATEs in Eucalyptus camaldulensis

Content Provider	Springer Nature Link
Author	Sawaki, Yoshiharu Kihara Doi, Tomori Kobayashi, Yuriko Nishikubo, buyuki Kawazu, Tetsu Kobayashi, Yasufumi Koyama, Hiroyuki Sato, Shigeru
Copyright Year	2012
Abstract	Many plant species excrete organic acids into the rhizosphere in response to aluminum stress to protect sensitive cells from aluminum rhizotoxicity. When the roots of Eucalyptus camaldulensis, a major source of pulp production, were incubated in aluminum-toxic medium, citrate released into the solution increased as a function of time. Citrate excretion was inducible by aluminum, but not by copper or sodium chloride stresses. This indicated that citrate is the major responsive organic acid released from the roots of this plant species to protect the root tips from aluminum damage. Four genes highly homologs to known citrate-transporting multidrugs and toxic compounds exclusion proteins, named EcMATE1–4, were isolated using polymerase chain reaction-based cloning techniques. Their predicted proteins included 12 membrane spanning domains, a common structural feature of citrate-transporting MATE proteins, and consisted of 502–579 amino acids with >60 % homology to orthologous genes in other plant species. One of the homologs, designated EcMATE1, was expressed in the roots more abundantly than in the shoots and in response to both Al and low pH stresses. Ectopic expression of EcMATE1 and 3 in tobacco hairy roots enhanced Al-responsive citrate excretion. Pharmacological characterization indicated that Al-responsive citrate excretion involved a protein phosphorylation/dephosphorylation process. These results indicate that citrate excretion through citrate-transporting multidrugs and toxic compounds exclusion proteins is one of the important aluminum-tolerance mechanisms in Eucalyptus camaldulensis.
Starting Page	979
Ending Page	989
Page Count	11
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	237
Issue Number	4
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-11-28
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Acid soil Aluminum tolerance Citrate excretion Eucalyptus camaldulensis Plant Sciences Agriculture Ecology Forestry
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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