NDLI: Conversion of membrane lipid acyl groups to triacylglycerol and formation of lipid bodies upon nitrogen starvation in biofuel green algae Chlorella UTEX29

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Conversion of membrane lipid acyl groups to triacylglycerol and formation of lipid bodies upon nitrogen starvation in biofuel green algae Chlorella UTEX29

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Planta : Volume 238, Issue 4, October 2013

Planta : Volume 238, Issue 3, September 2013

Planta : Volume 238, Issue 2, August 2013

Planta : Volume 238, Issue 1, July 2013

Conversion of membrane lipid acyl groups to triacylglycerol and formation of lipid bodies upon nitrogen starvation in biofuel green algae Chlorella UTEX29

Content Provider	Springer Nature Link
Author	Goncalves, Elton C. Johnson, Jodie V. Rathinasabapathi, Bala
Copyright Year	2013
Abstract	Algal lipids are ideal biofuel sources. Our objective was to determine the contributors to triacylglycerol (TAG) accumulation and lipid body formation in Chlorella UTEX29 under nitrogen (N) deprivation. A fivefold increase in intracellular lipids following N starvation for 24 h confirmed the oleaginous characteristics of UTEX29. Ultrastructural studies revealed increased number of lipid bodies and decreased starch granules in N-starved cells compared to N-replete cells. Lipid bodies were observed as early as 3 h after N removal and plastids collapsed after 48 h of stress. Moreover, the identification of intracellular pyrenoids and differences in the expected nutritional requirements for Chlorella protothecoides (as UTEX29 is currently classified) led us to conduct a phylogenetic study using 18S and actin cDNA sequences. This indicated UTEX29 to be more phylogenetically related to Chlorella vulgaris. To investigate the fate of different lipids after N starvation, radiolabeling using 14C-acetate was used. A significant decrease in 14C-galactolipids and phospholipids matched the increase in 14C-TAG starting at 3 h of N starvation, consistent with acyl groups from structural lipids as sources for TAG under N starvation. These results have important implications for the identification of key steps controlling oil accumulation in N-starved biofuel algae and demonstrate membrane recycling during lipid body formation.
Starting Page	895
Ending Page	906
Page Count	12
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	238
Issue Number	5
e-ISSN	14322048
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-08-09
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biofuel Chlorella Galactolipid Radiolabeling Triacylglycerol Ultrastructure Plant Sciences Agriculture Ecology Forestry
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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