NDLI: Characterization of abiotic stress-responsive Arabidopsis thaliana RD29A and RD29B genes and evaluation of transgenes

Please wait, while we are loading the content...

Isolation and expression analysis of partial sequences of heavy metal transporters from Brassica juncea by coupling high throughput cloning with a molecular fingerprinting technique

Down-regulation of the myo-inositol oxygenase gene family has no effect on cell wall composition in Arabidopsis

The roles of ascorbic acid and glutathione in symptom alleviation to SA-deficient plants infected with RNA viruses

Laticifer proteins play a defensive role against hemibiotrophic and necrotrophic phytopathogens

Physiological consequences of desiccation in the aquatic bryophyte Fontinalis antipyretica

Oviposition by a moth suppresses constitutive and herbivore-induced plant volatiles in maize

Characterization of abiotic stress-responsive Arabidopsis thaliana RD29A and RD29B genes and evaluation of transgenes

Content Provider	Springer Nature Link
Author	Msanne, Joseph Lin, Jiusheng Stone, Julie M. Awada, Tala
Copyright Year	2011
Abstract	Abiotic stresses have adverse effects on plant growth and productivity. The homologous RD29A and RD29B genes are exquisitely sensitive to various abiotic stressors. Therefore, RD29A and RD29B gene sequences have potential to confer abiotic stress resistance in crop species grown in arid and semi-arid regions. To our knowledge, no information on the physiological roles of the proteins encoded by RD29A and RD29B are available in the literature. To understand how these proteins function, we used reverse genetic approaches, including identifying rd29a and rd29b T-DNA knockout mutants, and examining the effects of complementing transgenes with the genes under control of their native promoters and chimeric genes with the native promoters swapped. Four binary vectors with the RD29A and RD29B promoters upstream of the cognate RD29A and RD29B cDNAs and as chimeric genes with noncognate promoters were used to transform rd29a and rd29b plants. Cold, drought, and salt induced both genes; the promoter of RD29A was found to be more responsive to drought and cold stresses, whereas the promoter of RD29B was highly responsive to salt stress. Morphological and physiological responses of rd29a and rd29b plants to salt stress were further investigated. Root growth, and photosynthetic properties declined significantly, while solute concentration (Ψπ), water use efficiency (WUE) and δ13C ratio increased under salt stress. Unexpectedly, the rd29a and rd29b knockout mutant lines maintained greater root growth, photosynthesis, and WUE under salt stress relative to control. We conclude that the RD29A and RD29B proteins are unlikely to serve directly as protective molecules.
Starting Page	97
Ending Page	107
Page Count	11
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	234
Issue Number	1
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-03-04
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Abiotic stress RD29 Water use efficiency Gas exchange Photosynthesis Carbon isotope ratio Forestry Ecology Agriculture Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Analysis and application of RD29 genes in abiotic stress response

Activity of the Arabidopsis RD29A and RD29B promoter elements in soybean under water stress

Brassinosteroid confers tolerance in Arabidopsis thaliana and Brassica napus to a range of abiotic stresses

Morphological and physiological variation in western redcedar (Thuja plicata) populations under contrasting soil water conditions

Seasonal gas exchange and water relations in juveniles of two evergreen Neotropical savanna tree species with contrasting regeneration strategies

In silico selection of Arabidopsis thaliana ecotypes with enhanced stress tolerance.

Changes in water use with growth in Ulmus pumila in semiarid sandy land of northern China

Carbon Isotope Discrimination, Gas Exchange and Stem Growth of Four Euramerican Hybrid Poplars under Different Watering Regimes

Transcriptome analyses of Arabidopsis thaliana seedlings grown in space: implications for gravity-responsive genes

Characterization of abiotic stress-responsive Arabidopsis thaliana RD29A and RD29B genes and evaluation of transgenes

Similar Documents

Analysis and application of RD29 genes in abiotic stress response

Activity of the Arabidopsis RD29A and RD29B promoter elements in soybean under water stress

Brassinosteroid confers tolerance in Arabidopsis thaliana and Brassica napus to a range of abiotic stresses

Morphological and physiological variation in western redcedar (Thuja plicata) populations under contrasting soil water conditions

Seasonal gas exchange and water relations in juveniles of two evergreen Neotropical savanna tree species with contrasting regeneration strategies

In silico selection of Arabidopsis thaliana ecotypes with enhanced stress tolerance.

Changes in water use with growth in Ulmus pumila in semiarid sandy land of northern China

Carbon Isotope Discrimination, Gas Exchange and Stem Growth of Four Euramerican Hybrid Poplars under Different Watering Regimes

Transcriptome analyses of Arabidopsis thaliana seedlings grown in space: implications for gravity-responsive genes

Characterization of abiotic stress-responsive Arabidopsis thaliana RD29A and RD29B genes and evaluation of transgenes