NDLI: Glucose sensing and the mitochondrial alternative pathway are involved in the regulation of astaxanthin biosynthesis in the dark-grown Chlorella zofingiensis (Chlorophyceae)

Content Provider	Springer Nature Link
Author	Li, Yantao Huang, Junchao Sandmann, Gerhard Chen, Feng
Copyright Year	2008
Abstract	The biosynthesis of the ketocarotenoid astaxanthin is a subject of scientific and industrial interest. The unicellular green alga Chlorella zofingiensis Dönz is able to grow and accumulate astaxanthin in the dark with exogenous glucose as sole carbon and energy source. In this study, the transcription of β-carotenoid ketolase (BKT) and β-carotenoid hydroxylase (CHYb) genes were surveyed to reveal the regulation of astaxanthin biosynthesis in dark-grown Chlorella zofingiensis. Coupled with glucose analogs and the hexokinase inhibitor glucosamine, we found that phosphorylation of glucose (glucose sensing) was essential to the increased transcription of BKT and CHYb genes and the accumulation of astaxanthin in the dark-grown cells. However, phosphorylation of glucose per se only up-regulated the transcription of CHYb and stimulated the synthesis of zeaxanthin. Blockage of the mitochondrial alternative pathway eliminated the glucose effects on the increased transcription of BKT and astaxanthin accumulation, suggesting that signals from alternative pathway was involved in the up-regulation of BKT transcription. In addition, citrate was shown to up-regulate the transcription of BKT independent of reactive oxygen species formation. Taken altogether, we conclude that in dark-grown Chlorella zofingiensis, the transcription of BKT and CHYb genes are differently regulated by the metabolism of glucose, through which the biosynthesis of astaxanthin is regulated.
Starting Page	735
Ending Page	743
Page Count	9
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	228
Issue Number	5
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-07-03
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Astaxanthin Carotenoid ketolase Chlorella Glucose sensing Respiratory electron transport TCA cycle acid Forestry Ecology Agriculture Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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