NDLI: Identification and characterization of HTD2: a novel gene negatively regulating tiller bud outgrowth in rice

Content Provider	Springer Nature Link
Author	Liu, Wenzhen Wu, Chao Fu, Yaping Hu, Guocheng Si, Huamin Zhu, Li Luan, Weijiang He, Zhengquan Sun, Zongxiu
Copyright Year	2009
Abstract	Tiller number is highly regulated by controlling the formation of tiller bud and its subsequent outgrowth in response to endogenous and environmental signals. Here, we identified a rice mutant htd2 from one of the 15,000 transgenic rice lines, which is characterized by a high tillering and dwarf phenotype. Phenotypic analysis of the mutant showed that the mutation did not affect formation of tiller bud, but promoted the subsequent outgrowth of tiller bud. To isolate the htd2 gene, a map-based cloning strategy was employed and 17 new insertions-deletions (InDels) markers were developed. A high-resolution physical map of the chromosomal region around the htd2 gene was made using the F2 and F3 population. Finally, the gene was mapped in 12.8 kb region between marker HT41 and marker HT52 within the BAC clone OSJNBa0009J13. Cloning and sequencing of the target region from the mutant showed that the T-DNA insertion caused a 463 bp deletion between the promoter and first exon of an esterase/lipase/thioesterase family gene in the 12.8 kb region. Furthermore, transgenic rice with reduced expression level of the gene exhibited an enhanced tillering and dwarf phenotype. Accordingly, the esterase/lipase/thioesterase family gene (TIGR locus Os03g10620) was identified as the HTD2 gene. HTD2 transcripts were expressed mainly in leaf. Loss of function of HTD2 resulted in a significantly increased expression of HTD1, D10 and D3, which were involved in the strigolactone biosynthetic pathway. The results suggest that the HTD2 gene could negatively regulate tiller bud outgrowth by the strigolactone pathway.
Starting Page	649
Ending Page	658
Page Count	10
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	230
Issue Number	4
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-07-05
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	HTD2 High tillering Dwarf Map-based cloning T-DNA Rice (Oryza sativa L.) Ecology Forestry Agriculture Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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