NDLI: CHUP1 mediates actin-based light-induced chloroplast avoidance movement in the moss Physcomitrella patens

Content Provider	Springer Nature Link
Author	Usami, Hiroka Maeda, Takuma Fujii, Yusuke Oikawa, Kazusato Takahashi, Fumio Kagawa, Takatoshi Wada, Masamitsu Kasahara, Masahiro
Copyright Year	2012
Abstract	Chloroplasts change their intracellular distribution in response to light intensity. CHUP1 (CHLOROPLAST UNUSUAL POSITIONING1) is indispensable for this response in Arabidopsis thaliana. However, involvement of CHUP1 in light-induced chloroplast movement is unknown in other plants. In this study, CHUP1 orthologues were isolated from a moss, Physcomitrella patens, and a fern, Adiantum capillus-veneris, by cDNA library screening and PCR cloning based on the P. patens genome sequence. Functional motifs found in CHUP1 of A. thaliana were conserved among the CHUP1 orthologues. In addition to the putative functional regions, the C-terminal regions (approximately 250 amino acids), which are unique in CHUP1s, were highly conserved. Green fluorescent protein (GFP) fusions of P. patens CHUP1s (PpCHUP1A, PpCHUP1B and PpCHUP1C) were transiently expressed in protoplast cells. All GFP fusions were localized on the chloroplasts. Light-induced chloroplast avoidance movement of chup1 disruptants of P. patens was examined in the presence of cytoskeletal inhibitors because of the utilization of both microtubules and actin filaments for the movement in P. patens. When actin filaments were disrupted by cytochalasin B, the wild type (WT) and all chup1 disruptants showed chloroplast avoidance movement. However, when microtubules were disrupted by Oryzalin, chloroplasts in ∆chup1A and ∆chup1A/B rarely moved and stayed in the strong light-irradiated area. On the other hand, WT, ∆chup1B and ∆chup1C showed chloroplast avoidance movement. These results suggest that PpCHUP1A predominantly mediates the actin-based light-induced chloroplast avoidance movement. This study reveals that CHUP1 functions on the chloroplasts and is involved in the actin-based light-induced chloroplast avoidance movement in P. patens.
Starting Page	1889
Ending Page	1897
Page Count	9
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	236
Issue Number	6
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-08-30
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Actin filaments Blue light Chloroplast movement CHUP1 Physcomitrella Ecology Plant Sciences Forestry Agriculture
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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