NDLI: A novel plastidial lipoxygenase of maize (Zea mays) ZmLOX6 encodes for a fatty acid hydroperoxide lyase and is uniquely regulated by phytohormones and pathogen infection

Content Provider	Springer Nature Link
Author	Gao, Xiquan Stumpe, Michael Feussner, Ivo Kolomiets, Michael
Copyright Year	2007
Abstract	Lipoxygenases (LOXs) are members of a large enzyme family that catalyze oxygenation of free polyunsaturated fatty acids into diverse hydroperoxide compounds, collectively called oxylipins. Although LOXs have been well studied in dicot species, reports of the genes encoding these enzymes are scarce for monocots, especially maize. Herein, we reported the cloning, characterization and molecular functional analysis of a novel maize LOX gene, ZmLOX6. The ZmLOX6 nucleotide sequence encodes a deduced translation product of 892 amino acids. Phylogenetic analysis showed that ZmLOX6 is distantly related to previously reported 9- or 13-LOXs from maize and other plant species, including rice and Arabidopsis. Although sequence prediction suggested cytoplasmic localization of this protein, ZmLOX6 protein has been reportedly isolated from mesophyll cell chloroplasts, emphasizing the unique features of this protein. Plastidial localization was confirmed by chloroplast uptake experiments with the in vitro translated protein. Analysis of recombinant protein revealed that ZmLOX6 has lost fatty acid hydroperoxide forming activity but 13-LOX-derived fatty acid hydroperoxides were cleaved into odd-chain ω-oxo fatty acids and as yet not identified C5-compound. In line with its reported abundance in mesophyll cells, ZmLOX6 was predominantly expressed in leaf tissue. Northern blot analysis demonstrated that ZmLOX6 was induced by jasmonic acid, but repressed by abscisic acid, salicylic acid and ethylene and was not responsive to wounding or insects. Further, this gene was strongly induced by the fungal pathogen Cochliobolus carbonum during compatible interactions, suggesting that ZmLOX6 may contribute to susceptibility to this pathogen. The potential involvement of ZmLOX6 in maize interactions with pathogens is discussed.
Starting Page	491
Ending Page	503
Page Count	13
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	227
Issue Number	2
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-10-09
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Dioxygenase Lipoxygenase Oxylipins Plant–pathogen interaction Susceptibility factor Zea Forestry Ecology Agriculture Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

ZmCCD7/ZpCCD7 encodes a carotenoid cleavage dioxygenase mediating shoot branching

Comparative molecular and biochemical characterization of segmentally duplicated 9-lipoxygenase genes ZmLOX4 and ZmLOX5 of maize

Floral transition in maize infected with Sporisorium reilianum disrupts compatibility with this biotrophic fungal pathogen

Formation of oxylipins by CYP74 enzymes

GENOTYPE X ENVIRONMENT INTERACTION IN FORAGE MAIZE (Zea mays L.)

Application of the pathogen Trojan horse approach in maize (Zea mays)

Genome-wide identification and phylogenetic analysis of Family-1 UDP glycosyltransferases in maize (Zea mays)

The core effector Cce1 is required for early infection of maize by Ustilago maydis

Differential distribution of the lipoxygenase pathway enzymes within potato chloroplasts

A novel plastidial lipoxygenase of maize (Zea mays) ZmLOX6 encodes for a fatty acid hydroperoxide lyase and is uniquely regulated by phytohormones and pathogen infection

Similar Documents

ZmCCD7/ZpCCD7 encodes a carotenoid cleavage dioxygenase mediating shoot branching

Comparative molecular and biochemical characterization of segmentally duplicated 9-lipoxygenase genes ZmLOX4 and ZmLOX5 of maize

Floral transition in maize infected with Sporisorium reilianum disrupts compatibility with this biotrophic fungal pathogen

Formation of oxylipins by CYP74 enzymes

GENOTYPE X ENVIRONMENT INTERACTION IN FORAGE MAIZE (Zea mays L.)

Application of the pathogen Trojan horse approach in maize (Zea mays)

Genome-wide identification and phylogenetic analysis of Family-1 UDP glycosyltransferases in maize (Zea mays)

The core effector Cce1 is required for early infection of maize by Ustilago maydis

Differential distribution of the lipoxygenase pathway enzymes within potato chloroplasts

A novel plastidial lipoxygenase of maize (Zea mays) ZmLOX6 encodes for a fatty acid hydroperoxide lyase and is uniquely regulated by phytohormones and pathogen infection