NDLI: Sweet pepper ferredoxin-like protein (pflp) gene as a novel selection marker for orchid transformation

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Tla1, a DNA insertional transformant of the green alga Chlamydomonas reinhardtii with a truncated light-harvesting chlorophyll antenna size

Sweet pepper ferredoxin-like protein (pflp) gene as a novel selection marker for orchid transformation

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Sweet pepper ferredoxin-like protein (pflp) gene as a novel selection marker for orchid transformation

Content Provider	Springer Nature Link
Author	You, Su Juan Liau, Chia Hui Huang, Hsiang En Feng, Teng Yung Prasad, Venkatesh Hsiao, Hsin hao Lu, Jian Cheng Chan, Ming Tsair
Copyright Year	2003
Abstract	A novel method for selection of transgenic plants utilizing the sweet pepper (Capsicum annuum L.) ferredoxin-like protein (pflp) gene as selection marker and Erwinia carotovora as the selection agent has been developed. An expression vector containing a pflp cDNA driven by a cauliflower mosaic virus 35S promoter was successfully transformed into protocorm-like bodies of Oncidium orchid by Agrobacterium tumefaciens and particle bombardment, respectively. Erwinia carotovora was used as a selection agent to screen transformants, thereby obtaining transgenic plants without the use of an antibiotic selection agent. A total of 32 independent transgenic orchid lines were obtained, out of which 9 transgenic lines (β-glucuronidase positive) were randomly selected and confirmed by Southern and northern blot analyses. The transgenic orchid plants showed enhanced resistance to E. carotovora, even when the entire plant was challenged with the pathogen. Our results suggest the novel use of the pflp gene as a resistance selection marker in plant genetic engineering strategies. In the future, the use of the pflp gene as a selection marker may facilitate the use of smaller gene constructs due to removal of bulky antibiotic selection and reporter genes. These constructs can then be used to incorporate additional genes of choice.
Starting Page	60
Ending Page	65
Page Count	6
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	217
Issue Number	1
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-02-12
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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