NDLI: Chloroplast-targeted expression of synthetic cry1Ac in transgenic rice as an alternative strategy for increased pest protection

Content Provider	Springer Nature Link
Author	Kim, Eun Hye Suh, Seok Cheol Park, Beom Seok Shin, Kong Sik Kweon, Soon Jong Han, Eun Jung Park, Su Hyun Kim, Youn Shic Kim, Ju Kon
Copyright Year	2009
Abstract	To increase insect resistance in transgenic rice plants, a synthetic truncated cry1Ac gene was linked to the rice rbcS promoter and its transit peptide sequence (tp) for chloroplast-targeted expression. Several transgenic lines were generated by the Agrobacterium-mediated transformation method and the expression levels of the transgene were compared with untargeted expression. Use of the rbcS-tp sequence increased the cry1Ac transcript and protein levels by 25- and 100-fold, respectively, with the accumulated protein in chloroplasts comprising up to 2% of the total soluble proteins. The high level of cry1Ac expression resulted in high levels of plant resistance to three common rice pests, rice leaf folder, rice green caterpillar, and rice skipper, as evidenced by insect feeding assays. Transgenic plants were also evaluated for resistance to natural infestations by rice leaf folder under field conditions. Throughout the entire period of plant growth, the transgenic plants showed no symptoms of damage, whereas nontransgenic control plants were severely damaged by rice leaf folders. Our results demonstrate that the targeting of cry1Ac protein to the chloroplast using the rbcS:tp system confers a high level of plant protection to insects, thus providing an alternative strategy for crop insect management.
Starting Page	397
Ending Page	405
Page Count	9
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	230
Issue Number	2
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-05-30
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chloroplast targeting Insect resistance rbcS transit peptide Synthetic cry1Ac Transgenic rice Ecology Forestry Agriculture Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Homologous promoter derived constitutive and chloroplast targeted expression of synthetic cry1Ac in transgenic chickpea confers resistance against Helicoverpa armigera

Compositional comparative analysis between insect-resistant rice (Oryza sativa L.) with a synthetic cry1Ac gene and its non-transgenic counterpart

Characterization of the rice RbcS3 promoter and its transit peptide for use in chloroplast-targeted expression

Molecular characterization of lepidopteran pest-resistant transgenic rice events expressing synthetic Cry1Ac

Enhanced resistance to the rice blast fungus Magnaporthe grisea conferred by expression of a cecropin A gene in transgenic rice

Chloroplast-targeted expression of the codon-optimized truncated cry1Ah gene in transgenic tobacco confers a high level of protection against insects

Evaluation of transgenic lines of Indian mustard (Brassica juncea L. Czern and Coss) expressing synthetic cry1Ac gene for resistance to Plutella xylostella

Increased pathogen resistance and yield in transgenic plants expressing combinations of the modified antimicrobial peptides based on indolicidin and magainin

Chloroplast targeting signal of a rice rbcS gene enhances transgene expression

Chloroplast-targeted expression of synthetic cry1Ac in transgenic rice as an alternative strategy for increased pest protection

Similar Documents

Homologous promoter derived constitutive and chloroplast targeted expression of synthetic cry1Ac in transgenic chickpea confers resistance against Helicoverpa armigera

Compositional comparative analysis between insect-resistant rice (Oryza sativa L.) with a synthetic cry1Ac gene and its non-transgenic counterpart

Characterization of the rice RbcS3 promoter and its transit peptide for use in chloroplast-targeted expression

Molecular characterization of lepidopteran pest-resistant transgenic rice events expressing synthetic Cry1Ac

Enhanced resistance to the rice blast fungus Magnaporthe grisea conferred by expression of a cecropin A gene in transgenic rice

Chloroplast-targeted expression of the codon-optimized truncated cry1Ah gene in transgenic tobacco confers a high level of protection against insects

Evaluation of transgenic lines of Indian mustard (Brassica juncea L. Czern and Coss) expressing synthetic cry1Ac gene for resistance to Plutella xylostella

Increased pathogen resistance and yield in transgenic plants expressing combinations of the modified antimicrobial peptides based on indolicidin and magainin

Chloroplast targeting signal of a rice rbcS gene enhances transgene expression

Chloroplast-targeted expression of synthetic cry1Ac in transgenic rice as an alternative strategy for increased pest protection