NDLI: Monoclonal antibodies indicate low-abundance links between heteroxylan and other glycans of plant cell walls

Content Provider	Springer Nature Link
Author	Cornuault, Valérie Buffetto, Fanny Rydahl, Maja G. Marcus, Susan E. Torode, Thomas A. Xue, Jie Crépeau, Marie Jeanne Faria Blanc, Nu Willats, William G. T. Dupree, Paul Ralet, Marie Christine Kx, J. Paul
Copyright Year	2015
Abstract	The derivation of two sensitive monoclonal antibodies directed to heteroxylan cell wall polysaccharide preparations has allowed the identification of potential inter-linkages between xylan and pectin in potato tuber cell walls and also between xylan and arabinogalactan-proteins in oat grain cell walls. Plant cell walls are complex composites of structurally distinct glycans that are poorly understood in terms of both in muro inter-linkages and developmental functions. Monoclonal antibodies (MAbs) are versatile tools that can detect cell wall glycans with high sensitivity through the specific recognition of oligosaccharide structures. The isolation of two novel MAbs, LM27 and LM28, directed to heteroxylan, subsequent to immunisation with a potato cell wall fraction enriched in rhamnogalacturonan-I (RG-I) oligosaccharides, is described. LM27 binds strongly to heteroxylan preparations from grass cell walls and LM28 binds to a glucuronosyl-containing epitope widely present in heteroxylans. Evidence is presented suggesting that in potato tuber cell walls, some glucuronoxylan may be linked to pectic macromolecules. Evidence is also presented that suggests in oat spelt xylan both the LM27 and LM28 epitopes are linked to arabinogalactan-proteins as tracked by the LM2 arabinogalactan-protein epitope. This work extends knowledge of the potential occurrence of inter-glycan links within plant cell walls and describes molecular tools for the further analysis of such links.
Starting Page	1321
Ending Page	1334
Page Count	14
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	242
Issue Number	6
e-ISSN	14322048
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-07-25
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Arabinogalactan-proteins Cell wall Glucuronoxylan Pectin Polysaccharides Rhamnogalacturonan-I Plant Sciences Agriculture Ecology Forestry
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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Monoclonal antibodies indicate low-abundance links between heteroxylan and other glycans of plant cell walls

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