NDLI: Vibrational microspectroscopy enables chemical characterization of single pollen grains as well as comparative analysis of plant species based on pollen ultrastructure

Content Provider	Springer Nature Link
Author	Zimmermann, Boris Bağcıoğlu, Murat Sandt, Christophe Kohler, Achim
Copyright Year	2015
Abstract	Chemical imaging of pollen by vibrational microspectroscopy enables characterization of pollen ultrastructure, in particular phenylpropanoid components in grain wall for comparative study of extant and extinct plant species. A detailed characterization of conifer (Pinales) pollen by vibrational microspectroscopy is presented. The main problems that arise during vibrational measurements were scatter and saturation issues in Fourier transform infrared (FTIR), and fluorescence and penetration depth issues in Raman. Single pollen grains larger than approx. 15 µm can be measured by FTIR microspectroscopy using conventional light sources, while smaller grains may be measured by employing synchrotron light sources. Pollen grains that were larger than 50 µm were too thick for FTIR imaging since the grain constituents absorbed almost all infrared light. Chemical images of pollen were obtained on sectioned samples, unveiling the distribution and concentration of proteins, carbohydrates, sporopollenins and lipids within pollen substructures. The comparative analysis of pollen species revealed that, compared with other Pinales pollens, Cedrus atlantica has a higher relative amount of lipid nutrients, as well as different chemical composition of grain wall sporopollenin. The pre-processing and data analysis, namely extended multiplicative signal correction and principal component analysis, offer simple estimate of imaging spectral data and indirect estimation of physical properties of pollen. The vibrational microspectroscopy study demonstrates that detailed chemical characterization of pollen can be obtained by measurement of an individual grain and pollen ultrastructure. Measurement of phenylpropanoid components in pollen grain wall could be used, not only for the reconstruction of past environments, but for assessment of diversity of plant species as well. Therefore, analysis of extant and extinct pollen species by vibrational spectroscopies is suggested as a valuable tool in biology, ecology and palaeosciences.
Starting Page	1237
Ending Page	1250
Page Count	14
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	242
Issue Number	5
e-ISSN	14322048
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-08-20
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	FTIR microspectroscopy Raman microspectroscopy Pinales Imaging Cell wall Plant Sciences Agriculture Ecology Forestry
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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