NDLI: Neutral invertases in grapevine and comparative analysis with Arabidopsis, poplar and rice

Content Provider	Springer Nature Link
Author	nis, Alberto Ruperti, Benedetto Pierasco, Alessandro Canaguier, Aurelie Adam Blondon, Anne Françoise Di Gaspero, Gabriele Vizzotto, Giannina
Copyright Year	2008
Abstract	Neutral invertases (NIs, EC 3.2.1.26) cleave sucrose to glucose and fructose. They are encoded by a small gene family of 9 members in the Arabidopsis genome, 8 in rice, 16 in poplar and 9 in Vitis vinifera (L.). The grapevine NIs were identified in the 8.4X genome assembly of the quasi-homozygous line PN40024. In addition, alleles of three NIs were sequenced in the heterozygous cultivar ‘Cabernet Sauvignon’. Analyses of sequence variation between alleles, homoeologous and paralogous copies in grapevine and their orthologues in Arabidopsis, poplar and rice are provided. In grapevine, NIs were classified into four α NIs and five β NIs and subsequently grouped into hierarchical clades using a combination of evidence including amino acid identity, exon/intron structure, rate of synonymous substitutions (K s) and chromosomal distribution. Estimation of K s proved the ancient origin of all NIs and the lack of expansion by gene duplication past the event of polyploidisation. We then focused on transcription analysis of five NIs for which evidence of expression was available from expressed sequence tag databases. Among these, four NIs consisted of pairs of homoeologous copies, each pair lying on a pair of chromosomes duplicated by polyploidy. Unequal expression of homoeologous genes was observed by quantitative RT-PCR in leaf, flower, seed and root tissues. Since NIs might play significant roles in fruit and wine quality, NIs expression was monitored in flesh and skin of ‘Merlot’ berries and shown in parallel with the suite of changes that accompany fruit ripening, including glucose and fructose accumulation.
Starting Page	129
Ending Page	142
Page Count	14
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	229
Issue Number	1
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-09-18
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Expression divergence Fruit development Homoeologous genes Ripening Vitis vinifera Ecology Forestry Agriculture Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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