NDLI: Localization of actin filaments on mitotic apparatus in tobacco BY-2 cells

Content Provider	Springer Nature Link
Author	Yasuda, Hiroshi Kanda, Katsuhiro Koiwa, Hiroyuki Suenaga, Kayoko Kidou, Shin ichiro Ejiri, Shin ichiro
Copyright Year	2005
Abstract	Actin filaments are among the major components of the cytoskeleton, and participate in various cellular dynamic processes. However, conflicting results had been obtained on the localization of actin filaments on the mitotic apparatus and their participation in the process of chromosome segregation. We demonstrated by using rhodamine-phalloidin staining, the localization of actin filaments on the mitotic spindles of tobacco BY-2 cells when the cells were treated with cytochalasin D. At prophase, several clear spots were observed at or near the kinetochores of the chromosomes. At anaphase, the actin filaments that appeared to be pulling chromosomes toward the division poles were demonstrated. However, as there was a slight possibility that these results might have been the artifacts of cytochalasin D treatment or the phalloidin staining, we analyzed the localization of actin filaments at the mitotic apparatus immunologically. We cloned a novel BY-2 α-type actin cDNA and prepared a BY-2 actin antibody. The fluorescence of the anti-BY-2 actin antibody was clearly observed at the mitotic apparatus in both non-treated and cytochalasin D-treated BY-2 cells during mitosis. The facts that similar results were obtained in both actin staining with rhodamine-phalloidin and immunostaining with actin antibody strongly indicate the participation of actin in the organization of the spindle body or in the process of chromosome segregation. Furthermore, both filamentous actin and spindle bodies disappeared in the cells treated with propyzamide, which depolymerizes microtubules, supporting the notion that actin filaments are associated with microtubules organizing the spindle body.
Starting Page	118
Ending Page	129
Page Count	12
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	222
Issue Number	1
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-04-27
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Actin filament BY-2 cell Cytochalasin D Spindle body Mitosis Nicotiana tabacum Plant Sciences Agriculture Ecology Forestry
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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