NDLI: Conditional modulation of NAD levels and metabolite profiles in Nicotiana sylvestris by mitochondrial electron transport and carbon/nitrogen supply

Content Provider	Springer Nature Link
Author	Hager, Jutta Pellny, Till K. Mauve, Caroline Lelarge Trouverie, Caroline Paepe, Rosine Foyer, Christine H. ctor, Graham
Copyright Year	2010
Abstract	Environmental controls on leaf NAD status remain poorly understood. Here, we analyzed the effects of two key environmental variables, CO2 and nitrogen, on leaf metabolite profiles, NAD status and the abundance of key transcripts involved in de novo NAD synthesis in wild-type (WT) Nicotiana sylvestris and the CMSII mutant that lacks respiratory complex I. High CO2 and increased N supply both significantly enhanced NAD+ and NADH pools in WT leaves. In nitrogen-sufficient conditions, CMSII leaves were enriched in NAD+ and NADH compared to the WT, but the differences in NADH were smaller at high CO2 than in air because high CO2 increased WT NADH/NAD+. The CMSII-linked increases in NAD+ and NADH status were abolished by growth with limited nitrogen, which also depleted the nicotine and nicotinic acid pools in the CMSII leaves. Few statistically significant genotype and N-dependent differences were detected in NAD synthesis transcripts, with effects only on aspartate oxidase and NAD synthetase mRNAs. Non-targeted metabolite profiling as well as quantitative amine analysis showed that NAD+ and NADH contents correlated tightly with leaf amino acid contents across all samples. The results reveal considerable genotype- and condition-dependent plasticity in leaf NAD+ and NADH contents that is not linked to modified expression of NAD synthesis genes at the transcript level and show that NAD+ and NADH contents are tightly integrated with nitrogen metabolism. A regulatory two-way feedback circuit between nitrogen and NAD in the regulation of N assimilation is proposed that potentially links the nutritional status to NAD-dependent signaling pathways.
Starting Page	1145
Ending Page	1157
Page Count	13
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	231
Issue Number	5
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-02-25
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Nucleotides NAD synthesis Photorespiration Redox GC–MS Ecology Forestry Agriculture Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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