NDLI: In-vitro selection of Vitis vinifera `Chardonnay' with Elsinoe ampelina culture filtrate is accompanied by fungal resistance and enhanced secretion of chitinase

Please wait, while we are loading the content...

In-vitro selection of Vitis vinifera `Chardonnay' with Elsinoe ampelina culture filtrate is accompanied by fungal resistance and enhanced secretion of chitinase

Increases in cytosolic Ca2+ are not required for abscisic acid-inhibition of inward K+ currents in guard cells of Vicia faba L.

Altered exopolysaccharides of Bradyrhizobium japonicum mutants correlate with impaired soybean lectin binding, but not with effective nodule formation

Developing prolamine protein bodies are associated with the cortical cytoskeleton in rice endosperm cells

Biosynthesis of spermidine, a direct precursor of pyrrolizidine alkaloids in root cultures of Senecio vulgaris L.

PH regulation in apoplastic and cytoplasmic cell compartments of leaves

Identification and partial characterization of proteins and proteoglycans encrusting the secondary cell walls of flax fibres

Aberrant chloroplasts in transgenic rice plants expressing a high level of maize NADP-dependent malic enzyme

Evidence for covalent linkage between xyloglucan and acidic pectins in suspension-cultured rose cells

The negative effect of nitrate on gametogenesis is independent of nitrate assimilation in Chlamydomonas reinhardtii

Units, symbols, abbreviations

Planta : Volume 211, Issue 1, June 2000

In-vitro selection of Vitis vinifera `Chardonnay' with Elsinoe ampelina culture filtrate is accompanied by fungal resistance and enhanced secretion of chitinase

Content Provider	Springer Nature Link
Author	Jayasankar, S. Li, Zhijian Gray, D. J.
Copyright Year	2000
Abstract	Proembryogenic masses of grapevine (Vitis vinifera L.) `Chardonnay' (clone 02Ch) were exposed to the culture filtrate of Elsinoe ampelina (deBary) Shear, the causal agent of anthracnose disease. After four or five cycles of recurrent in-vitro selection with medium containing 40% fungal culture filtrate, putative resistant lines RC 1 and RC 2 respectively, were established. The selected lines inhibited the growth of E. ampelina and Fusarium oxysporium (Schlecht.) (isolated from watermelon) in a dual-culture assay and reduced the growth of mycelium on a conditioned-medium test, thus suggesting the involvement of extracellular compounds in resistance. Sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) gel electrophoresis of extracellular proteins from spent suspension-culture medium showed enhanced secretion of new proteins by selected lines. A 36-kDa protein was immunodetected by a chitinase antiserum. This chitinase continued to express constitutively in differentiated somatic embryos and also in the intercellular fluids of plants regenerated from the selected lines. Somatic embryos from selected lines grew uninhibitedly in a medium containing 40% fungal culture filtrate, whereas non-selected (control) somatic embryos became necrotic and died within a few days. Plants regenerated from selected lines exhibited resistance to infection by E. ampelina in both greenhouse tests and detached leaf bioassays. Results suggest that embryogenic cells can be selected for resistance following in-vitro selection, resulting in resistant plants. Whether or not resistant cells pre-existed in the original embryogenic culture or were induced by the selection pressure could not be determined.
Starting Page	200
Ending Page	208
Page Count	9
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	211
Issue Number	2
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2000-07-14
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Characterization of nine Mlo family genes and analysis of their expression against pathogen infections in Vitis flexuosa

Studies On Anthracnose Disease Of Grapevine (Vitis Vinifera L.) Caused By Elsinoe Ampelina (De Bary) Shear

Identification and Expression Profiles of Six Transcripts Encoding Carboxylesterase Protein in Vitis flexuosa Infected with Pathogens

Evaluation of grapevine varietal resistance to anthracnose through treating culture filtrates from Elsinoe ampelina

The effect of the orientation of stem segments of grapevine (Vitis vinifera) cv. Chardonnay on callus development in vitro

Extracellular compounds produced by fungi associated with Botryosphaeria dieback induce differential defence gene expression patterns and necrosis in Vitis vinifera cv. Chardonnay cells

Vitis vinifera VvNPR1.1 is the functional ortholog of AtNPR1 and its overexpression in grapevine triggers constitutive activation of PR genes and enhanced resistance to powdery mildew

Combined elicitation of chitosan and ultraviolet C enhanced stilbene production and expression of chitinase and β-1,3-glucanase in Vitis vinifera cell suspension cultures

Biotransformation of C$_{13}$-norisoprenoids and monoterpenes by a cell suspension culture of cv. Gamay (Vitis vinifera)

In-vitro selection of Vitis vinifera `Chardonnay' with Elsinoe ampelina culture filtrate is accompanied by fungal resistance and enhanced secretion of chitinase

Similar Documents

Characterization of nine Mlo family genes and analysis of their expression against pathogen infections in Vitis flexuosa

Studies On Anthracnose Disease Of Grapevine (Vitis Vinifera L.) Caused By Elsinoe Ampelina (De Bary) Shear

Identification and Expression Profiles of Six Transcripts Encoding Carboxylesterase Protein in Vitis flexuosa Infected with Pathogens

Evaluation of grapevine varietal resistance to anthracnose through treating culture filtrates from Elsinoe ampelina

The effect of the orientation of stem segments of grapevine (Vitis vinifera) cv. Chardonnay on callus development in vitro

Extracellular compounds produced by fungi associated with Botryosphaeria dieback induce differential defence gene expression patterns and necrosis in Vitis vinifera cv. Chardonnay cells

Vitis vinifera VvNPR1.1 is the functional ortholog of AtNPR1 and its overexpression in grapevine triggers constitutive activation of PR genes and enhanced resistance to powdery mildew

Combined elicitation of chitosan and ultraviolet C enhanced stilbene production and expression of chitinase and β-1,3-glucanase in Vitis vinifera cell suspension cultures

Biotransformation of C$_{13}$-norisoprenoids and monoterpenes by a cell suspension culture of cv. Gamay (Vitis vinifera)

In-vitro selection of Vitis vinifera `Chardonnay' with Elsinoe ampelina culture filtrate is accompanied by fungal resistance and enhanced secretion of chitinase