NDLI: Ryanodine receptors and the mediation of Ca2+-dependent anion secretion across rat colon

Content Provider	Springer Nature Link
Author	Kocks, Sarah Schultheiss, Gerhard Diener, Martin
Copyright Year	2002
Abstract	The properties of the Na+–Ca2+ exchanger in isolated crypts from rat colon were studied using the Fura-2 imaging technique. The transport mode of the exchanger was reversed by replacing extracellular Na+ by the impermeable cation, N-methyl-D-glucamine (NMDG+), so that the transporter mediated a Ca2+ influx into the cells. Depletion of intracellular Ca2+ stores by inhibitors of sarcoplasmatic endoplasmatic calcium ATPases (SERCA), i.e., cyclopiazonic acid (10–5 mol l–1) or thapsigargin (10–6 mol l–1), reduced the increase in [Ca2+]i evoked by superfusion with NMDG+, suggesting a cross-talk between the exchanger and the Ca2+ stores. However, measurement of Ca2+ influx with the Mn2+ quench technique revealed that the activity of the exchanger was independent of the filling state of the stores. Instead, the obvious inhibition of the [Ca2+]i response by SERCA blockers was due to a reduction of Ca2+-induced Ca2+ release after inhibition of store-refilling. The functional presence of ryanodine receptors was demonstrated by the increase in [Ca2+]i evoked by ryanodine (10–7 to 3×10–4 mol l–1) in a concentration-dependent manner. This effect was mimicked by cADP ribose (10–5 mol l–1) in crypts permeabilized with saponin (10 mg l–1). Ruthenium red (5×10–5 mol l–1) or high concentrations of ryanodine (3×10–4 mol l–1) inhibited this response. In Ussing chamber experiments ruthenium red (5×10–4 mol l–1) or a high concentration of ryanodine (10–3 mol l–1) inhibited the increase in short-circuit current evoked by the cholinergic agonist, carbachol (5×10–5 mol l–1). Consequently, Ca2+-induced Ca2+ release may act as kind of amplifier during Ca2+-dependent Cl– secretion in order to maintain a long-lasting increase in the intracellular Ca2+ concentration.
Starting Page	390
Ending Page	397
Page Count	8
File Format	PDF
ISSN	00316768
Journal	Pflügers Archiv
Volume Number	445
Issue Number	3
e-ISSN	14322013
Language	English
Publisher	Springer-Verlag
Publisher Date	2002-10-08
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Clinical Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Ryanodine receptors and the mediation of Ca2+-dependent anion secretion across rat colon

Effects of dopamine on ion transport across the rat distal colon

Fatty acids inhibit anion secretion in rat colon: apical and basolateral action sites

Losartan antagonism of angiotensin-II-induced potassium secretion across rat colon

Stimulation of colonic anion secretion by monochloramine: action sites

Modal gating transitions in cardiac ryanodine receptors during increases of Ca2+ concentration produced by photolysis of caged Ca2+

Rapid cooling-induced contractures in rat skinned skeletal muscle fibres originate from sarcoplasmic reticulum Ca2+ release through ryanodine and inositol trisphosphate receptors

Ryanodine receptor type 3 does not contribute to contractions in the mouse myometrium regardless of pregnancy

Modulation of Ca2+ release through ryanodine receptors in vascular smooth muscle by protein kinase Cα

Ryanodine receptors and the mediation of Ca2+-dependent anion secretion across rat colon

Similar Documents

Ryanodine receptors and the mediation of Ca2+-dependent anion secretion across rat colon

Effects of dopamine on ion transport across the rat distal colon

Fatty acids inhibit anion secretion in rat colon: apical and basolateral action sites

Losartan antagonism of angiotensin-II-induced potassium secretion across rat colon

Stimulation of colonic anion secretion by monochloramine: action sites

Modal gating transitions in cardiac ryanodine receptors during increases of Ca2+ concentration produced by photolysis of caged Ca2+

Rapid cooling-induced contractures in rat skinned skeletal muscle fibres originate from sarcoplasmic reticulum Ca2+ release through ryanodine and inositol trisphosphate receptors

Ryanodine receptor type 3 does not contribute to contractions in the mouse myometrium regardless of pregnancy

Modulation of Ca2+ release through ryanodine receptors in vascular smooth muscle by protein kinase Cα

Ryanodine receptors and the mediation of Ca2+-dependent anion secretion across rat colon