NDLI: Inhibition of mitochondrial function affects cellular Ca2+ handling in pancreatic B-cells

Content Provider	Springer Nature Link
Author	Düfer, Martina Krippeit Drews, Peter Drews, Gisela
Copyright Year	2002
Abstract	The mitochondrial inhibitors NaN3 and carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone (FCCP) were used to study the role of mitochondria in pancreatic B-cell Ca2+ homeostasis. In glucose-stimulated B-cells NaN3 and FCCP both increased the KATP current and thus hyperpolarized the cell membrane potential, as expected for agents depleting cellular ATP. NaN3 and FCCP stopped the glucose-induced oscillations in the cytosolic free Ca2+ concentration ([Ca2+]c) and elicited a biphasic response. After a first rapid and transient increase, [Ca2+]c rose in a second slow phase to a sustained level. In cells pretreated with thapsigargin the first inhibitor-induced rise in [Ca2+]c was absent, suggesting that it may be due to Ca2+ mobilization from intracellular stores. The glucose-induced oscillations were terminated again by NaN3 and FCCP, respectively, but the slow increase in [Ca2+]c of the second phase was still present. A minute increase in [Ca2+]c elicited by NaN3 or FCCP was even visible after the removal of extracellular Ca2+, suggesting that the inhibitors also mobilize Ca2+ from mitochondria. NaN3 and FCCP induced Ca2+ influx into B-cells treated with low glucose concentrations whose voltage-dependent Ca2+ channels are closed. Experiments with thapsigargin-preincubated cells indicate that disturbance of mitochondrial function stimulates Ca2+ influx through voltage-independent Ca2+ pathways. During the NaN3-induced increase in [Ca2+]c, K+-elicited depolarizations of the cells did not further augment [Ca2+]c. Evidently, this is due to a direct inhibitory effect of azide on L-type Ca2+ channels. The data demonstrate that disturbing the mitochondrial function affects cellular Ca2+ homeostasis in B-cells at several sites. Thus, it is concluded that intact mitochondrial function is a prerequisite for regular Ca2+ handling in B-cells.
Starting Page	236
Ending Page	243
Page Count	8
File Format	PDF
ISSN	00316768
Journal	Pflügers Archiv
Volume Number	444
Issue Number	1-2
e-ISSN	14322013
Language	English
Publisher	Springer-Verlag
Publisher Date	2014-03-19
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Human Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Clinical Biochemistry

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