NDLI: Basolateral localization of flounder Na+-dicarboxylate cotransporter (fNaDC-3) in the kidney of Pleuronectes americanus

Content Provider	Springer Nature Link
Author	Hentschel, Hartmut Burckhardt, Birgitta C. Schölermann, Beate Kühne, Lars Burckhardt, Gerhard Steffgen, Jürgen
Copyright Year	2003
Abstract	The purpose of this study was to provide functional and immunocytochemical evidence for the location of the winter flounder (Pleuronectes americanus) sodium-dicarboxylate cotransporter-3 (fNaDC-3) in the basolateral membrane of proximal tubule cells. fNaDC-3 was expressed in Xenopus laevis oocytes. Lowering the external pH from 7.5 to 6.5 or 5.5 modestly decreased the uptake of [14C]succinate into fNaDC-3 expressing oocytes, but markedly increased the uptake of [14C]citrate. As measured by the two-electrode voltage-clamp technique, the citrate concentration eliciting half-maximal current, K 0.5, decreased from 490 µM at pH 7.5 to 32 µM at pH 6.0. The maximal inwards current, ΔI max, increased from −27 to −72 nA, when bath pH was changed from 7.5 to 6.0. These data suggest that fNaDC-3 translocates preferably divalent citrate. cis-Aconitate, a tricarboxylate that interacts exclusively with basolateral sodium-dicarboxylate cotransport in the rat kidney, was translocated by fNaDC-3 with a K 0.5 of 300 µM. Antibodies raised against an NaDC-3-specific peptide reacted with the basal cell side of flounder renal proximal tubule segment II (PII). No other structures were stained, indicating that fNaDC-3 is located exclusively in the basolateral membrane of PII cells. We assume that fNaDC-3 provides PII cells with Krebs cycle intermediates as fuels and with α-ketoglutarate to drive organic anion secretion.
Starting Page	578
Ending Page	584
Page Count	7
File Format	PDF
ISSN	00316768
Journal	Pflügers Archiv
Volume Number	446
Issue Number	5
e-ISSN	14322013
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-05-21
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Succinate transport Immunostaining pH dependence Basolateral Citrate cis-Aconitate Flounder
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Clinical Biochemistry

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