NDLI: Ca2+ clearance by plasmalemmal NCLX, Li+-permeable Na+/Ca2+ exchanger, is required for the sustained exocytosis in rat insulinoma INS-1 cells

Content Provider	Springer Nature Link
Author	Han, Young Eun Ryu, Shin Young Park, Sun Hyun Lee, Kyu Hee Lee, Suk Ho Ho, Won Kyung
Copyright Year	2015
Abstract	Na+/Ca2+ exchangers are key players for Ca2+ clearance in pancreatic β-cells, but their molecular determinants and roles in insulin secretion are not fully understood. In the present study, we newly discovered that the Li+-permeable Na+/Ca2+ exchangers (NCLX), which were known as mitochondrial Na+/Ca2+ exchangers, contributed to the Na+-dependent Ca2+ movement across the plasma membrane in rat INS-1 insulinoma cells. Na+/Ca2+ exchange activity by NCLX was comparable to that by the Na+/Ca2+ exchanger, NCX. We also confirmed the presence of NCLX proteins on the plasma membrane using immunocytochemistry and cell surface biotinylation experiments. We further investigated the role of NCLX on exocytosis function by measuring the capacitance increase in response to repetitive depolarization. Small interfering (si)RNA-mediated downregulation of NCLX did not affect the initial exocytosis, but significantly suppressed sustained exocytosis and recovery of exocytosis. XIP (NCX inhibitory peptide) or Na+ replacement for inhibiting Na+-dependent Ca2+ clearance also selectively suppressed sustained exocytosis. Consistent with the idea that sustained exocytosis requires ATP-dependent vesicle recruitment, mitochondrial function, assessed by mitochondrial membrane potential (ΔΨ), was impaired by siNCLX or XIP. However, depolarization-induced exocytosis was hardly affected by changes in intracellular Na+ concentration, suggesting a negligible contribution of mitochondrial Na+/Ca2+ exchanger. Taken together, our data indicate that Na+/Ca2+ exchanger-mediated Ca2+ clearance mediated by NCLX and NCX is crucial for optimizing mitochondrial function, which in turn contributes to vesicle recruitment for sustained exocytosis in pancreatic β-cells.
Starting Page	2461
Ending Page	2472
Page Count	12
File Format	PDF
ISSN	00316768
Journal	Pflügers Archiv
Volume Number	467
Issue Number	12
e-ISSN	14322013
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-06-24
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ca2+ transport Na+/Ca2+ exchanger Exocytosis Capacitance Pancreatic β-cell Human Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Clinical Biochemistry

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