NDLI: Properties of the inward-rectifying Cl– channel in rat choroid plexus: regulation by intracellular messengers and inhibition by divalent cations

Content Provider	Springer Nature Link
Author	Kajita, Hidetoshi Whitwell, Caroline Brown, Peter D.
Copyright Year	2000
Abstract	The properties of the inward-rectifying Cl– conductance in rat choroid plexus epithelial cells were investigated to allow comparisons to be made with ClC-2. All experiments were performed using the whole-cell configuration of the patch-clamp method. The conductance was transiently activated using an electrode solution which contained 375 nM catalytic subunit of protein kinase A (PKA). PKA failed to activate the conductance, however, when cells were pre-incubated with phorbol esters, which activate protein kinase C [1 µM phorbol 12-myristate 13-acetate (PMA) and 1 µM phorbol 12,13-dibutyrate (PDBu)]. Sustained activation of the conductance by PKA was observed in Ca2+-free conditions (5 mM BAPTA in the electrode solution), or when 100 nM calphostin C, a PKC inhibitor, was added to the electrode solution. The inward-rectifying Cl– conductance in choroid plexus is therefore similar to ClC-2 in that it is inhibited by PKC. The inward-rectifying conductance was blocked when Cd2+ (30 and 300 µM) and Zn2+ (1, 30 and 300 µM) were added to the bath solution. ClC-2 channels are also blocked by Zn2+ and Cd2+. The magnitude of the inward conductance was dependent on the concentration of ATP in the electrode solution. The conductance was not observed when ATP in the electrode was replaced with non-hydrolysable ATP analogues {adenosine 5′-O-(3-thiotriphosphate) (ATP[γ-S]) and 5′-adenylylimidodiphosphate (AMP-PNP)}, but it was supported by UTP and GTP. These data contrast with those of previous studies in which ClC-2 channels were activated in the absence of ATP. In conclusion, the inward-rectifying Cl– channel in rat choroid plexus shares some properties with ClC-2 (inhibition by PKC and block by divalent cations), but differs in that it depends on intracellular ATP.
Starting Page	933
Ending Page	940
Page Count	8
File Format	PDF
ISSN	00316768
Journal	Pflügers Archiv
Volume Number	440
Issue Number	6
e-ISSN	14322013
Language	English
Publisher	Springer-Verlag
Publisher Date	2000-07-26
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Clinical Biochemistry

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