NDLI: Anoctamins support calcium-dependent chloride secretion by facilitating calcium signaling in adult mouse intestine

Content Provider	Springer Nature Link
Author	Schreiber, Rainer Faria, Diana Skryabin, Boris V. Wanitchakool, Podchanart Rock, Jason R. Kunzelmann, Karl
Copyright Year	2014
Abstract	Intestinal epithelial electrolyte secretion is activated by increase in intracellular cAMP or Ca2+ and opening of apical Cl− channels. In infants and young animals, but not in adults, Ca2+-activated chloride channels may cause secretory diarrhea during rotavirus infection. While detailed knowledge exists concerning the contribution of cAMP-activated cystic fibrosis transmembrane conductance regulator (CFTR) channels, analysis of the role of Ca2+-dependent Cl− channels became possible through identification of the anoctamin (TMEM16) family of proteins. We demonstrate expression of several anoctamin paralogues in mouse small and large intestines. Using intestinal-specific mouse knockout models for anoctamin 1 (Ano1) and anoctamin 10 (Ano10) and a conventional knockout model for anoctamin 6 (Ano6), we demonstrate the role of anoctamins for Ca2+-dependent Cl− secretion induced by the muscarinic agonist carbachol (CCH). Ano1 is preferentially expressed in the ileum and large intestine, where it supports Ca2+-activated Cl− secretion. In contrast, Ano10 is essential for Ca2+-dependent Cl− secretion in jejunum, where expression of Ano1 was not detected. Although broadly expressed, Ano6 has no role in intestinal cholinergic Cl− secretion. Ano1 is located in a basolateral compartment/membrane rather than in the apical membrane, where it supports CCH-induced Ca2+ increase, while the essential and possibly only apical Cl− channel is CFTR. These results define a new role of Ano1 for intestinal Ca2+-dependent Cl− secretion and demonstrate for the first time a contribution of Ano10 to intestinal transport.
Starting Page	1203
Ending Page	1213
Page Count	11
File Format	PDF
ISSN	00316768
Journal	Pflügers Archiv
Volume Number	467
Issue Number	6
e-ISSN	14322013
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-07-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	TMEM16A TMEM16F TMEM16K Anoctamin 1 Anoctamin 6 Anoctamin 10 Ano1 Ano6 Ano10 Ca2+-activated Cl− channels Colon Small intestine Ileum Jejunum chloride secretion Human Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Clinical Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Expression of anoctamins in retinal pigment epithelium (RPE)

Paneth Cell Secretion in vivo Requires Expression of Tmem16a and Tmem16f.

Anoctamins are a family of Ca2+-activated Cl− channels

Regulation of TMEM16A/ANO1 and TMEM16F/ANO6 ion currents and phospholipid scrambling by $Ca^{2+}$and plasma membrane lipid

The role of TMEM16A (ANO1) and TMEM16F (ANO6) in cell migration

Molecular mechanisms of activation and regulation of ANO1-Encoded Ca²⁺-Activated Cl^- channels.

The role of TMEM16A (ANO1) and TMEM16F (ANO6) in cell migration

Cl− channels in apoptosis

Expression and Function of Epithelial Anoctamins

Anoctamins support calcium-dependent chloride secretion by facilitating calcium signaling in adult mouse intestine

Similar Documents

Expression of anoctamins in retinal pigment epithelium (RPE)

Paneth Cell Secretion in vivo Requires Expression of Tmem16a and Tmem16f.

Anoctamins are a family of Ca2+-activated Cl− channels

Regulation of TMEM16A/ANO1 and TMEM16F/ANO6 ion currents and phospholipid scrambling by $Ca^{2+}$and plasma membrane lipid

The role of TMEM16A (ANO1) and TMEM16F (ANO6) in cell migration

Molecular mechanisms of activation and regulation of ANO1-Encoded Ca2+-Activated Cl- channels.

The role of TMEM16A (ANO1) and TMEM16F (ANO6) in cell migration

Cl− channels in apoptosis

Expression and Function of Epithelial Anoctamins

Anoctamins support calcium-dependent chloride secretion by facilitating calcium signaling in adult mouse intestine

Molecular mechanisms of activation and regulation of ANO1-Encoded Ca²⁺-Activated Cl^- channels.