NDLI: Changes in the mechanical and adhesive behaviour of human neutrophils on cooling in vitro

Content Provider	Springer Nature Link
Author	Nash, Gerard B. Abbitt, Katherine B. Tate, Karen Jetha, Karim A. Egginton, Stuart
Copyright Year	2001
Abstract	Changes in the rheological properties of neutrophils may influence flow in microvessels that are cooled below normal body temperature. We investigated the effects of temperature on the mechanical and adhesive properties of human neutrophils by measuring transit times for individual cells flowing through 8-µm-pores in filters, and adhesion to P-selectin for cells perfused over a monolayer of activated platelets. Pore transit time increased as temperature was decreased from 37°C to 0°C. Upon rapid cooling, there was an instantaneous increase attributable to changes in aqueous viscosity. Interestingly, at 10°C specifically, there was an additional increase in transit time, which was abolished by the inhibitor of actin polymerization, cytochalasin B. This meant that by 15 min, transit time at 10°C was greater than at 0°C. Most adherent cells on P-selectin were rolling, rather than stationary, at 10, 26 or 37°C. The velocity of rolling slowed with decreasing temperature. The total number of adherent cells decreased with increasing wall shear rate, but for a given shear rate there was relatively little effect of temperature on attachment. However, when adhesion at 10, 26 or 37°C was compared at equal shear stress (taking into account fluid viscosity), adhesion was greatest at 10°C. Measurements of immunofluorescence showed that exposure to 10°C gradually increased expression of β2-integrin CD11b/CD18, but this did not cause transformation to stationary adhesion with time in the flow assay. Thus, neutrophils show an anomalous rheological response around 10°C, which may impair local microcirculation in the cold. On rewarming, "activated" cells might inhibit recovery or become released into the systemic circulation.
Starting Page	762
Ending Page	770
Page Count	9
File Format	PDF
ISSN	00316768
Journal	Pflügers Archiv
Volume Number	442
Issue Number	5
e-ISSN	14322013
Language	English
Publisher	Springer-Verlag
Publisher Date	2001-06-02
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Clinical Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Activity-dependent changes in excitability of perirhinal cortex networks in vitro

Changing world of neutrophils

Mechanical power and efficiency in running children

Gravity and thermoregulation: metabolic changes and circadian rhythms

Influence of adsorbed plasma proteins on erythrocyte rheological properties: in vitro and ex vivo studies

CAMP- and cGMP-independent stretch-induced changes in the contraction of rat atrium

Dynamic changes in phosphoinositide levels control ion channel activity

PH regulation in isolated in vitro perfused rat colonic crypts

Dynamic changes in sarcoplasmic reticulum function in cardiac hypertrophy and failure

Changes in the mechanical and adhesive behaviour of human neutrophils on cooling in vitro

Similar Documents

Activity-dependent changes in excitability of perirhinal cortex networks in vitro

Changing world of neutrophils

Mechanical power and efficiency in running children

Gravity and thermoregulation: metabolic changes and circadian rhythms

Influence of adsorbed plasma proteins on erythrocyte rheological properties: in vitro and ex vivo studies

CAMP- and cGMP-independent stretch-induced changes in the contraction of rat atrium

Dynamic changes in phosphoinositide levels control ion channel activity

PH regulation in isolated in vitro perfused rat colonic crypts

Dynamic changes in sarcoplasmic reticulum function in cardiac hypertrophy and failure

Changes in the mechanical and adhesive behaviour of human neutrophils on cooling in vitro