NDLI: Ionic mechanism of ouabain-induced swelling of leech Retzius neurons

Content Provider	Springer Nature Link
Author	Dierkes, Paul Wilhelm Wüsten, Hans Joachim Klees, Guido Müller, Anja Hochstrate, Peter
Copyright Year	2005
Abstract	By using electrophysiological and microfluorimetric methods, we found that leech Retzius neurons swell after inhibition of the Na+–K+ pump by the cardiac glycoside ouabain. To explore the mechanism of this swelling, we measured the effect of ouabain on [Na+]i, [K+]i, and [Cl−]i, as well as on the membrane potential, by applying triple-barrelled ion-sensitive microelectrodes. As shown previously, ouabain induced a marked [Na+]i increase, a [K+]i decrease, and a membrane depolarization, and it also evoked an increase in [Cl−]i. The analysis of the data revealed a net uptake of NaCl, which quantitatively explained the ouabain-induced cell swelling. In the absence of extracellular Na+ or Cl−, NaCl uptake was excluded, and the cell volume remained unaffected. Likewise, NaCl uptake and, hence, cell swelling did not occur when the Na+–K+ pump was inhibited by omitting bath K+. Also, in K+-free solution, [Na+]i increased and [K+]i dropped, but [Cl−]i slightly decreased, and after an initial, small membrane depolarization, the cells hyperpolarized for a prolonged period. It is concluded that the ouabain-induced NaCl uptake is caused by the depolarization of the plasma membrane, which augments the inwardly directed electrochemical Cl− gradient.
Starting Page	25
Ending Page	35
Page Count	11
File Format	PDF
ISSN	00316768
Journal	Pflügers Archiv
Volume Number	452
Issue Number	1
e-ISSN	14322013
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-12-10
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ouabain Cell volume Leech Ion-sensitive microelectrodes Fura-2 Retzius neuron Human Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Clinical Biochemistry

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