NDLI: Specific detection and semi-quantitative analysis of TRPC4 protein expression by antibodies

Content Provider	Springer Nature Link
Author	Flockerzi, Veit Jung, Christine Aberle, Thomas Meissner, Marcel Freichel, Marc Philipp, Stephan E. Nastainczyk, Wolfgang Maurer, Patrick Zimmermann, Richard
Copyright Year	2005
Abstract	In mouse tissues two variants of the transient receptor potential (canonical) (TRPC) 4 protein are expressed: the “full-length” TRPC4 protein and a slightly smaller variant, called TRPC4Δ761-864, which lacks 84 amino acid residues. Although the presence of mRNA encoding the TRPC4 protein in mammalian cells and the detection of the heterologously expressed TRPC4 protein by Western blot analysis have been reported, the unequivocal detection of endogenous TRPC4 proteins has proven difficult. In the present study we compared polyclonal antibodies for the detection of TRPC4 proteins in mouse tissues and monitored their specificity and reliability by analysing corresponding tissues from TRPC4-deficient mice. In addition we introduced a procedure that allows us to estimate the amount of TRPC4 protein expressed in a single cell. Using this technique it appears that the amount of TRPC4 protein expressed stably in HEK 293 cells is at least fourfold higher than the amount of TRPC4 protein expressed endogenously in the bovine adrenocortical cell line SBAC.
Starting Page	81
Ending Page	86
Page Count	6
File Format	PDF
ISSN	00316768
Journal	Pflügers Archiv
Volume Number	451
Issue Number	1
e-ISSN	14322013
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-06-18
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	TRP channels TRPC4 Cell-free synthesis Anti-TRPC antibody
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Clinical Biochemistry

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