NDLI: Sodium and chloride absorptive defects in the small intestine in Slc26a6 null mice

Content Provider	Springer Nature Link
Author	Seidler, Ursula Rottinghaus, Ingrid Hillesheim, Jutta Chen, Mingmin Riederer, Brigitte Krabbenhöft, Anja Engelhardt, Regina Wiemann, Martin Wang, Zhaouhui Barone, Sharon Manns, Michael P. Soleimani, Maocher
Copyright Year	2007
Abstract	PAT1 (Slc26a6) is located on the apical membrane of the small intestinal villi, but its role for salt absorption has not been studied. To ascertain the role of Slc26a6 in jejunal sodium and chloride absorption, and its interplay with NHE3, muscle-stripped jejuna from Slc26a6+/+ and −/− and NHE3 +/+ and −/− mice were mounted in Ussing chambers and electrical parameters, and 36Cl− and 22Na+ fluxes were measured. In parallel studies, expression of the apical Na+/H+ exchanger (NHE3) was examined by immunofluorescence labeling and immunoblot analysis in brush border membrane (BBM). In the basal state, net Cl− and Na+ fluxes were absorptive in Slc26a6−/− and +/+ jejuni, but significantly decreased in −/− animals. Upon forskolin addition, net Na+ absorption decreased, Isc strongly increased, and net Cl− flux became secretory in Slc26a6−/− and +/+ jejuni. When luminal glucose was added to activate Na+/glucose cotransport, concomitant Cl− absorption was significantly reduced in Slc26a6 −/− jejuni, while Na+ absorption increased to the same degree in Slc26a6 −/− and +/+ jejuni. Identical experiments in NHE3-deficient jejuni also showed reduced Na+ and Cl− absorption. Results further demonstrated that the lack of NHE3 rendered Na+ and Cl− absorption unresponsive to inhibition by cAMP, but did not affect glucose-driven Na+ and Cl− absorption. Immunoblotting revealed comparable NHE3 abundance and distribution in apical membranes in Slc26a6−/− and +/+ mice. The data strongly suggests that Slc26a6 acts in concert with NHE3 in electroneutral salt absorption in the small intestine. Slc26a6 also serves to absorb Cl− during glucose-driven salt absorption.
Starting Page	757
Ending Page	766
Page Count	10
File Format	PDF
ISSN	00316768
Journal	Pflügers Archiv
Volume Number	455
Issue Number	4
e-ISSN	14322013
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-09-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Electrolyte absorption Intestine Anion exchange Sodium absorption Chloride secretion Glucose absorption Human Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Clinical Biochemistry

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