NDLI: Transepithelial capacitance decrease reveals closure of lateral interspace in A6 epithelia

Content Provider	Springer Nature Link
Author	Van Driessche, W. De Vos, Rita Jans, Danny Simaels, Jeannine De Smet, Patrick Raskin, Gert
Copyright Year	1999
Abstract	A sine wave method was used to measure transepithelial capacitance (C T) at 4.1 kHz (C HF T ). Model calculations show that C HF T reflects the equivalent capacitance of the series arrangement of apical and basolateral membrane capacitance. Cell swelling induced by reducing the basolateral osmolality from 260 to 140 mosmol/kg H2O (NaCl or sucrose removal) transiently decreased C HF T . The decrease in C HF T (ΔC HF T ) reached its maximum 30 s after the onset of cell swelling and a complete recovery of C HF T was attained within 3–4 min. ΔC HF T could be diminished by manoeuvres that reduced the rate or amplitude of cell swelling, i.e. lowering the temperature or treatment with low concentrations of glutaraldehyde (0.025%). ΔC HF T increased with the magnitude of the osmotic perturbation but saturated at large volume expansions. ΔC HF T increased with culture time. Electron micrographs showed a clear correlation between time course of C HF T changes and the closure of the lateral interspace (LIS). A striking correlation between the occurrence of C HF T recovery and the ability of the cells to develop a regulatory volume decrease (RVD) was found: Gd3+ (0.5 mM) inhibited both phenomena. The frequency dependence of C T was obtained from impedance spectra recorded over the range of 4 Hz to 22 kHz. These data agree with model calculations in which the contribution of the access resistance to the lateral membrane was included. All observations are consistent with the idea that ΔC HF T originates from the closure of the LIS during cell swelling. The latter phenomenon increases the access resistance to the lateral membrane, which results in a marked reduction of the basolateral membrane area detected at high frequencies with capacitance measurements.
Starting Page	680
Ending Page	690
Page Count	11
File Format	PDF
ISSN	00316768
Journal	Pflügers Archiv
Volume Number	437
Issue Number	5
e-ISSN	14322013
Language	English
Publisher	Springer-Verlag
Publisher Date	1999-03-23
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Clinical Biochemistry

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