NDLI: On the mechanism of genistein-induced activation of protein kinase A-dependent Cl

Content Provider	Springer Nature Link
Author	Obayashi, Kazuhiko Horie, M. Washizuka, Takashi Nishimoto, Toshihisa Sasayama, Shigetake
Copyright Year	1999
Abstract	Genistein, an inhibitor of protein tyrosine kinase (PTK), enhanced the activation of the cardiac isoform of the protein kinase A (PKA)-regulated cystic fibrosis transmembrane conductance regulator (CFTR) Cl– conductance in guinea-pig ventricular cells. We examined the mechanism(s) underlying this excitatory action of genistein by using patch-clamp techniques. The CFTR Cl– conductance, activated by isoproterenol (ISO, 10 nM; [Cl–] 153 mM extracellular, 21 mM intracellular; 36 °C), was enhanced by 20 µM genistein. Daidzein, a structural analogue of genistein with little inhibitory action on PTK, also enhanced CFTR Cl– currents. After maximal activation of the Cl– conductance by a cocktail of adenosine 3’,5’-cyclic monophosphate, 3-isobutyl-1-methylxanthine and okadaic acid or vanadate plus forskolin in the pipette, genistein was no longer stimulatory but was rather slightly inhibitory at 100 µM. Direct exposure of myocytes to higher concentrations of genistein (50–100 µM) elicited outwardly rectifying currents with a reversal potential of –47 mV in the absence of ISO. In the presence of 50 µM H-89, a PKA inhibitor, genistein had no effect. Vanadate in the pipette at a concentration (100 µM) inhibiting phosphotyrosine phosphatases alone did not prevent the action of genistein. In contrast, no conductance was activated by tyrphostins B42 or 51 or lavendustin A, other PTK inhibitors. Genistein’s stimulation of cardiac CFTR Cl– conductance appears to be independent of the PTK pathway and to be due to its direct interaction with CFTR Cl– channels.
Starting Page	269
Ending Page	277
Page Count	9
File Format	PDF
ISSN	00316768
Journal	Pflügers Archiv
Volume Number	438
Issue Number	3
e-ISSN	14322013
Language	English
Publisher	Springer-Verlag
Publisher Date	1999-07-19
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Clinical Biochemistry

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