NDLI: Central leptin replacement enhances chemorespiratory responses in leptin-deficient mice independent of changes in body weight

Content Provider	Springer Nature Link
Author	Bassi, Mirian Giusti, Humberto Leite, Cristiane Mota Anselmo Franci, Janete A. Carmo, Jussara M. Silva, Alexandre A. Hall, John E. Colombari, Eduardo Glass, Mogens L.
Copyright Year	2012
Abstract	Previous studies showed that leptin-deficient (ob/ob) mice develop obesity and impaired ventilatory responses to CO2 $$ \left( {{{\dot{V}}_{{{\text{E}}\,}}}{ - }\,{\text{C}}{{\text{O}}_{{2}}}} \right) $$ . In this study, we examined if leptin replacement improves chemorespiratory responses to hypercapnia (7 % CO2) in ob/ob mice and if these effects were due to changes in body weight or to the direct effects of leptin in the central nervous system (CNS). $$ {\dot{V}_{{{\text{E}}\,}}}{\text{ - C}}{{\text{O}}_{{2}}} $$ was measured via plethysmography in obese leptin-deficient- (ob/ob) and wild-type- (WT) mice before and after leptin (10 μg/2 μl day) or vehicle (phosphate buffer solution) were microinjected into the fourth ventricle for four consecutive days. Although baseline $$ {\dot{V}_{\text{E}}} $$ was similar between groups, obese ob/ob mice exhibited attenuated $$ {\dot{V}_{{{\text{E}}\,}}}{ - }\,{\text{C}}{{\text{O}}_{{2}}} $$ compared to WT mice (134 ± 9 versus 196 ± 10 ml min−1). Fourth ventricle leptin treatment in obese ob/ob mice significantly improved $$ {\dot{V}_{{{\text{E}}\,}}}{ - }\,{\text{C}}{{\text{O}}_{{2}}} $$ (from 131 ± 15 to 197 ± 10 ml min−1) by increasing tidal volume (from 0.38 ± 0.03 to 0.55 ± 0.02 ml, vehicle and leptin, respectively). Subcutaneous leptin administration at the same dose administered centrally did not change $$ {\dot{V}_{{{\text{E}}\,}}}{ - }\,{\text{C}}{{\text{O}}_{{2}}} $$ in ob/ob mice. Central leptin treatment in WT had no effect on $$ {\dot{V}_{{{\text{E}}\,}}}{ - }\,{\text{C}}{{\text{O}}_{{2}}} $$ . Since the fourth ventricle leptin treatment decreased body weight in ob/ob mice, we also examined $$ {\dot{V}_{{{\text{E}}\,}}}{ - }\,{\text{C}}{{\text{O}}_{{2}}} $$ in lean pair-weighted ob/ob mice and found it to be impaired compared to WT mice. Thus, leptin deficiency, rather than obesity, is the main cause of impaired $$ {\dot{V}_{{{\text{E}}\,}}}{ - }\,{\text{C}}{{\text{O}}_{{2}}} $$ in ob/ob mice and leptin appears to play an important role in regulating chemorespiratory response by its direct actions on the CNS.
Starting Page	145
Ending Page	153
Page Count	9
File Format	PDF
ISSN	00316768
Journal	Pflügers Archiv
Volume Number	464
Issue Number	2
e-ISSN	14322013
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-05-15
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Respiratory chemoreception Obesity Hypercapnia Leptin Ventilation Human Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Clinical Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Central leptin replacement enhances chemorespiratory responses in leptin-deficient mice independent of changes in body weight

Serotonergic neurons in the nucleus raphe obscurus contribute to interaction between central and peripheral ventilatory responses to hypercapnia

Orexinergic system in the locus coeruleus modulates the CO2 ventilatory response

Human pulmonary vascular responses to hypoxia and hypercapnia

Leptin into the ventrolateral medulla facilitates chemorespiratory response in leptin-deficient (ob/ob) mice

Leptin into the ventrolateral medulla facilitates chemorespiratory response in leptin-deficient (ob/ob) mice

The role of pH-sensitive TASK channels in central respiratory chemoreception

Leptin-Independent Programming of Adult Body Weight and Adiposity in Mice

Chemosensory ventilatory responses in the mutant mice with Presbyterian hemoglobinopathy.

Central leptin replacement enhances chemorespiratory responses in leptin-deficient mice independent of changes in body weight

Similar Documents

Central leptin replacement enhances chemorespiratory responses in leptin-deficient mice independent of changes in body weight

Serotonergic neurons in the nucleus raphe obscurus contribute to interaction between central and peripheral ventilatory responses to hypercapnia

Orexinergic system in the locus coeruleus modulates the CO2 ventilatory response

Human pulmonary vascular responses to hypoxia and hypercapnia

Leptin into the ventrolateral medulla facilitates chemorespiratory response in leptin-deficient (ob/ob) mice

Leptin into the ventrolateral medulla facilitates chemorespiratory response in leptin-deficient (ob/ob) mice

The role of pH-sensitive TASK channels in central respiratory chemoreception

Leptin-Independent Programming of Adult Body Weight and Adiposity in Mice

Chemosensory ventilatory responses in the mutant mice with Presbyterian hemoglobinopathy.

Central leptin replacement enhances chemorespiratory responses in leptin-deficient mice independent of changes in body weight