NDLI: Ionic currents involved in shock-induced nonlinear changes in transmembrane potential responses of single cardiac cells

Please wait, while we are loading the content...

Novel adenoviral vectors coding for GFP-tagged wtCFTR and ΔF508-CFTR: characterization of expression and electrophysiological properties in A549 cells

Protein and gene expression of Ca2+ channel isoforms in murine colon: effect of inflammation

High glucose concentrations stimulate human monocyte sodium/hydrogen exchanger activity and modulate atherosclerosis-related functions

Concurrent measurements of the free cytosolic concentrations of H+ and Na+ ions with fluorescent indicators

Targeted in vivo expression of proteins in the calyx of Held

Pflügers Archiv : Volume 449, Issue 2, November 2004

Pflügers Archiv : Volume 449, Issue 1, October 2004

Pflügers Archiv : Volume 448

Pflügers Archiv : Volume 447

Pflügers Archiv : Volume 446

Pflügers Archiv : Volume 445

Pflügers Archiv : Volume 444

Pflügers Archiv : Volume 443

Pflügers Archiv : Volume 442

Pflügers Archiv : Volume 441

Pflügers Archiv : Volume 440

Pflügers Archiv : Volume 439

Pflügers Archiv : Volume 438

Pflügers Archiv : Volume 437

Pflügers Archiv : Volume 436

Pflügers Archiv : Volume 435

Pflügers Archiv : Volume 434

Pflügers Archiv : Volume 433

Ionic currents involved in shock-induced nonlinear changes in transmembrane potential responses of single cardiac cells

Content Provider	Springer Nature Link
Author	Sharma, Vid Tung, Leslie
Copyright Year	2004
Abstract	An exhaustive characterization of how an isolated cardiac cell responds to applied electric fields could serve as an important groundwork for understanding responses of more complex higher order systems. Field stimulation of single cardiac cells during the early plateau of the action potential results in a nonuniform change in transmembrane potential (Vm) across the cell length that is more heavily weighted in the negative direction. These negatively shifted Vm responses are not replicated theoretically using present day membrane models. The goal of this study was to explore the membrane currents involved in the field responses during the plateau by selectively blocking various ion channels. Enzymatically isolated single guinea pig cells were stimulated with uniform field S1-S2 pulses, and the transmembrane potential responses were optically recorded from several sites along the cell length to assess the drug effect. We used nine different pharmacological agents to manipulate the conductance of major cardiac ion channels of which only barium (Ba2+) altered the transmembrane potential responses. At 50 μM Ba2+, which specifically blocks inwardly rectifying current IK1, the negative shift in Vm responses was accentuated. At 1 mM Ba2+, which blocks both IK1 and sustained plateau current IKp, the negative shift diminished. However, 1 mM Ba2+ also depolarized the cells, and depressed or completely eliminated the action potential. Based on these results we conclude that IK1 contributes to field-induced responses during the plateau stimulation by passing a net inward current, which when blocked accentuates the negative shift in the Vm responses. A conclusive role of IKp could not be demonstrated because of confounding changes in membrane potential. However, from our results it remains as the most viable candidate for the elusive current that contributes a net outward current to produce negatively weighted Vm responses during plateau stimulation and warrants further investigation.
Starting Page	248
Ending Page	256
Page Count	9
File Format	PDF
ISSN	00316768
Journal	Pflügers Archiv
Volume Number	449
Issue Number	3
e-ISSN	14322013
Language	English
Publisher	Springer-Verlag
Publisher Date	2004-10-12
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Barium Drugs Optical mapping Potassium channels Voltage sensitive dye
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Clinical Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Analysis of field-induced transmembrane potential responses of single cardiac cells in terms of active and passive components

Zebrafish heart as a model for human cardiac electrophysiology

Decomposition of field-induced transmembrane potential responses of single cardiac cells

Zinc‐induced changes in ionic currents of clonal rat pancreatic β‐cells: activation of ATP‐sensitive K$ ^{+}$ channels

The voltage-dependent block of ATP-sensitive potassium channels of frog skeletal muscle by caesium and barium ions.

The Kv4.2 N-terminal restores fast inactivation and confers KChIP2 modulatory effects on N-terminal-deleted Kv1.4 channels

Zinc-induced changes in ionic currents of clonal rat pancreatic β-cells: activation of ATP-sensitive K+ channels

brane potential responses to high-intensity electrical shocks. Am J (2003)

Voltage clamp analysis of the effects of dopamine on the transmembrane ionic currents underlying the action potential of sheep cardiac Purkinje fibers.

Ionic currents involved in shock-induced nonlinear changes in transmembrane potential responses of single cardiac cells

Similar Documents

Analysis of field-induced transmembrane potential responses of single cardiac cells in terms of active and passive components

Zebrafish heart as a model for human cardiac electrophysiology

Decomposition of field-induced transmembrane potential responses of single cardiac cells

Zinc‐induced changes in ionic currents of clonal rat pancreatic β‐cells: activation of ATP‐sensitive K$ ^{+}$ channels

The voltage-dependent block of ATP-sensitive potassium channels of frog skeletal muscle by caesium and barium ions.

The Kv4.2 N-terminal restores fast inactivation and confers KChIP2 modulatory effects on N-terminal-deleted Kv1.4 channels

Zinc-induced changes in ionic currents of clonal rat pancreatic β-cells: activation of ATP-sensitive K+ channels

brane potential responses to high-intensity electrical shocks. Am J (2003)

Voltage clamp analysis of the effects of dopamine on the transmembrane ionic currents underlying the action potential of sheep cardiac Purkinje fibers.

Ionic currents involved in shock-induced nonlinear changes in transmembrane potential responses of single cardiac cells