NDLI: Silent but not dumb: how cellular trafficking and pore gating modulate expression of TWIK1 and THIK2

Content Provider	Springer Nature Link
Author	Bichet, Delphine Blin, Sandy Feliciangeli, Sylvain Chatelain, Franck C. Bobak, Nicole Lesage, Florian
Copyright Year	2014
Abstract	Among K2P channels, a few of them turned out to be difficult to express in heterologous systems and were coined “silent subunits”. Recent studies have shed light on the mechanisms behind this apparent lack of channel activity at the plasma membrane. For TWIK1 and THIK2 channels, silence is related to a combination of intracellular retention and low intrinsic activity. TWIK1 is constitutively endocytosed from the plasma membrane before being transported to recycling endosomes, whereas THIK2 is restricted to endoplasmic reticulum. These intracellular localizations are related to trafficking signals located in the cytoplasmic parts of the channels. When these motifs are mutated or masked, channels are redistributed at the plasma membrane and produce measurable currents. However, these currents are of modest amplitude. This weak basal activity is due to a hydrophobic barrier in the deep pore that limits water and ions in the conduction pathway. Other silent channels KCNK7, TWIK2, and TASK5 are still under study. Expression and characterization of these K2P channels pave the way for a better understanding of the mechanisms controlling intracellular trafficking of membrane proteins, ion conduction, and channel gating.
Starting Page	1121
Ending Page	1131
Page Count	11
File Format	PDF
ISSN	00316768
Journal	Pflügers Archiv
Volume Number	467
Issue Number	5
e-ISSN	14322013
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-10-24
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Potassium channels Ion selectivity Trafficking Gating Human Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Clinical Biochemistry

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Silent but not dumb: how cellular trafficking and pore gating modulate expression of TWIK1 and THIK2

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