NDLI: Supra-physiological doses of testosterone affect membrane oxidation of human neutrophils monitored by the fluorescent probe C11-BODIPY581/591

Content Provider	Springer Nature Link
Author	Souza Junior, Tácito Pessoa Yamada, André K. Simão, Roberto Polotow, Tatiana G. Curi, Rui Pope, Zachary Willardson, Jeffrey M. Barros, Marcelo P.
Copyright Year	2012
Abstract	The purpose of this study was to determine the effects of supra-physiological doses of testosterone (TES) on membrane oxidation of activated human neutrophils in vitro using an innovative and sensitive technique: the real-time detection with the fluorescence probe C11-BODIPY581/591. Methodological controls were performed with the lipid-soluble and powerful antioxidant astaxanthin at different neutrophil density cultures. Neutrophils from nine healthy young men (23.4 ± 2.5 years, 174.4 ± 7.0 cm height, and 78.3 ± 7.0 kg weight) were isolated and treated with 0.1 or 10 μM TES for 24 h and subsequently labeled with the free radical-sensitive probe C11-BODIPY581/591 for monitoring membrane oxidation after neutrophil activation with phorbol-12-myristate-13-acetate (PMA). First-order exponential decay kinetic indicated that both 0.1 and 10 μM TES severely increased baseline membrane oxidation in non-activated human neutrophils (compared to control). However, similar kinetics of membrane oxidation were observed in control and 0.1 μM TES-treated neutrophils after PMA activation, whereas chemical activation did not alter the baseline higher rates of membrane oxidation in 10 μM TES-treated neutrophils. The data presented here support the hypothesis that TES exerts distinct effects on the membrane oxidation of human neutrophils, depending on its dose (here, 102 to 104-fold higher than physiological levels in men) and on PMA activation of the oxidative burst. Furthermore, this paper also presents an innovative application of the free radical-sensitive probe C11-BODIPY581/591 for monitoring (auto-induced) membrane oxidation as an important parameter of viability and, thus, responsiveness of immune cells in inflammatory processes.
Starting Page	1241
Ending Page	1248
Page Count	8
File Format	PDF
ISSN	14396319
Journal	European Journal of Applied Physiology
Volume Number	113
Issue Number	5
e-ISSN	14396327
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-11-17
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Lipoperoxidation Steroid Astaxanthin Antioxidant Immune Oxidative stress Human Physiology Occupational Medicine/Industrial Medicine Sports Medicine
Content Type	Text
Resource Type	Article
Subject	Orthopedics and Sports Medicine Physiology (medical) Public Health, Environmental and Occupational Health Sports Science

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