NDLI: Lipid droplet changes in proliferating and quiescent 3T3 fibroblasts

Content Provider	Springer Nature Link
Author	Diaz, Giacomo Batetta, Barbara Sanna, Francesca Uda, Sabrina Reali, Camilla Angius, Fabrizio Melis, Marta Falchi, Angela Maria
Copyright Year	2008
Abstract	Lipid droplets (LDs) are fat-storing organelles present in virtually all eukaryotic cells and involved in many aspects of cell biology related to lipid metabolism and cholesterol homeostasis. In this study, we investigated the presence of LDs in proliferating and quiescent (contact-inhibited) 3T3 fibroblasts to verify a correlation with cell growth. LDs were characterized by Nile red staining, positivity to adipophilin and negativity to perilipin. LDs were numerous in proliferating cells, but very few in quiescent cells. However, the fraction of quiescent cells, which resumed proliferation after scratch-wound assay, also resumed the formation of LDs. In proliferating cells, the number of LDs correlated with the DNA content, suggesting a continuous accumulation of LDs during cell growth. These findings were supported by biochemical data showing much higher rates of cholesterol esterification and triglyceride synthesis in proliferating cells. Both filipin staining and the fluorescent cholesterol analog dehydroergosterol revealed the presence of an intense traffic of free cholesterol, mediated by acidic vesicles, in proliferating cells. Nile red ratiometric measurements revealed a different lipid composition of LDs in proliferating and quiescent cells. Changes in the number and composition of LDs were also found in growing cells treated with inhibitors of cholesterol esterification (Sandoz 58-035), endosomal cholesterol efflux (U18666A) and V-ATPase (bafilomycin-A1).
Starting Page	611
Ending Page	621
Page Count	11
File Format	PDF
ISSN	09486143
Journal	Histochemistry and Cell Biology
Volume Number	129
Issue Number	5
e-ISSN	1432119X
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-02-23
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cell growth Lipid droplets Cholesterol DHE 3T3 fibroblasts Medicine/Public Health Anatomy
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Molecular Biology Medical Laboratory Technology

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